{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T05:30:17Z","timestamp":1786685417912,"version":"build-2736575974"},"reference-count":42,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    To investigate the role and mechanism of METTL14\u2010mediated regulation of cuproptosis in myocardial ischemia\u2010reperfusion injury (MI\/RI). An MI\/RI rat model was established. H9C2 cells were cultured and subjected to hypoxia\/reoxygenation (H\/R) modeling. Cells were transfected with siRNAs targeting METTL14 and ATP7A. Cell viability was detected by CCK\u20108 assay. Intracellular Cu\n                    <jats:sup>2+<\/jats:sup>\n                    levels were measured using a biochemical kit. The interaction between METTL14 and ATP7A mRNA was verified by RNA immunoprecipitation (RIP) assay. Adenovirus containing si\u2010METTL14 was constructed and injected into rats, followed by the induction of the MI\/RI model. Cardiac function was evaluated by measuring ejection fraction (EF) and fractional shortening (FS) using echocardiography. Rat myocardial tissues were collected for Hematoxylin and Eosin (HE) staining. The mRNA expression of m6A regulatory enzymes was detected by quantitative PCR (qPCR). Cu\n                    <jats:sup>2+<\/jats:sup>\n                    levels in myocardial tissue were measured using a biochemical assay kit. The expression of cuproptosis\u2010related proteins was assessed by Western blot. MI\/RI rats showed significantly decreased FS and EF values, with necrosis and hemorrhage, increased Cu\n                    <jats:sup>2+<\/jats:sup>\n                    content, elevated mRNA levels of FTO, METTL3, ALKBH5, and METTL14, decreased ATP7A protein expression, and increased protein expression of FDX1, LIAS, and DLAT in the myocardial tissue. Intramyocardial injection of adenovirus containing si\u2010METTL14 reversed the MI\/RI\u2010induced changes. In vitro, decreased cell viability, increased Cu\n                    <jats:sup>2+<\/jats:sup>\n                    content, decreased ATP7A protein expression, and increased FDX1, LIAS, and DLAT protein expression were observed in H\/R stimulated H9C2 cells, which were remarkably rescued by si\u2010METTL14. Moreover, co\u2010transfection with both si\u2010METTL14 and si\u2010ATP7A reversed effects induced by si\u2010METTL14 alone. METTL14 promotes cuproptosis\u2010associated changes and exacerbates MI\/RI.\n                  <\/jats:p>","DOI":"10.1002\/jcb.70109","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T05:22:06Z","timestamp":1786684926000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["METTL14 Promotes Cuproptosis\u2010Associated Changes in Rats With Myocardial Ischemia\u2010Reperfusion Injury"],"prefix":"10.1002","volume":"127","author":[{"given":"Shengqiang","family":"Zeng","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Medicine The Second Affiliated Hospital of Nanchang University, Jiangxi Medical College, Nanchang University Nanchang Jiangxi China"},{"name":"Department of Cardiovascular Medicine Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dezhi","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine Jiangxi Provincial People's Hospital, The First Affiliated Hospital of Nanchang Medical College Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-0981-3910","authenticated-orcid":false,"given":"Yanqing","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine The Second Affiliated Hospital of Nanchang University, Jiangxi Medical College, Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"issue":"1","key":"e_1_2_12_2_1","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/s10020-021-00271-w","article-title":"ELAVL1 Is Transcriptionally Activated by FOXC1 and Promotes Ferroptosis in Myocardial Ischemia\/Reperfusion Injury by Regulating Autophagy","volume":"27","author":"Chen H. 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