{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:45:10Z","timestamp":1791917110692,"version":"4.3.4"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K07979"],"award-info":[{"award-number":["23K07979"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K10087"],"award-info":[{"award-number":["24K10087"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Cellular Biochemistry"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Adipogenin (\n                    <jats:italic>Adig<\/jats:italic>\n                    ) is a lipid droplet\u2013associated protein that promotes lipid droplet growth. CCAAT\/enhancer\u2010binding protein \u03b1 (C\/EBP\u03b1) is a transcription factor enriched in white adipose tissue and liver and is a key regulator of hepatic gene expression. In this study, we investigated the molecular mechanism by which C\/EBP\u03b1 regulates\n                    <jats:italic>Adig<\/jats:italic>\n                    gene expression in the liver of type 2 diabetic model (\n                    <jats:italic>ob\/ob<\/jats:italic>\n                    ) mice. Although hepatic C\/EBP\u03b1 expression levels were comparable between control and\n                    <jats:italic>ob\/ob<\/jats:italic>\n                    mice, both\n                    <jats:italic>Adig<\/jats:italic>\n                    mRNA levels and the putative ADIG signal were significantly elevated in the fatty liver of\n                    <jats:italic>ob\/ob<\/jats:italic>\n                    mice. This induction of\n                    <jats:italic>Adig<\/jats:italic>\n                    was markedly repressed by liver\u2010specific C\/EBP\u03b1 deficiency. Analysis of publicly available chromatin immunoprecipitation sequencing (ChIP\u2010seq) data revealed a C\/EBP\u03b1\u2010binding peak within the\n                    <jats:italic>Adig<\/jats:italic>\n                    promoter region in the mouse liver. Within this peak, we identified a putative C\/EBP response element (\n                    <jats:italic>Adig<\/jats:italic>\n                    \u2010CEBPRE, located at positions \u221287 to \u221279) that was relatively well conserved across multiple species, suggesting an evolutionarily conserved role of C\/EBP\u03b1 in\n                    <jats:italic>Adig<\/jats:italic>\n                    regulation. Reporter assays demonstrated that luciferase activity driven by\n                    <jats:italic>Adig<\/jats:italic>\n                    \u2010CEBPRE was significantly induced by either C\/EBP\u03b1 or C\/EBP\u03b2, whereas this induction was abolished upon deletion of\n                    <jats:italic>Adig<\/jats:italic>\n                    \u2010CEBPRE. Collectively, these findings indicate that\n                    <jats:italic>Adig<\/jats:italic>\n                    is a novel C\/EBP\u03b1 target gene in the steatotic liver of\n                    <jats:italic>ob\/ob<\/jats:italic>\n                    mice, providing a mechanistic link between hepatic transcriptional regulation and lipid droplet homeostasis.\n                  <\/jats:p>","DOI":"10.1002\/jcb.70134","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T17:49:53Z","timestamp":1791913793000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Adipogenin Is A Novel Target Gene for CCAAT\/Enhancer\u2010Binding Protein \u03b1"],"prefix":"10.1002","volume":"127","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3437-1531","authenticated-orcid":false,"given":"Daisuke","family":"Aibara","sequence":"first","affiliation":[{"name":"Faculty of Pharmaceutical Sciences Fukuoka University Fukuoka Fukuoka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimihiko","family":"Matsusue","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences Fukuoka University Fukuoka Fukuoka 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