{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T04:24:17Z","timestamp":1787199857700,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T00:00:00Z","timestamp":1733356800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004045","name":"Karadeniz Teknik \u00dcniversitesi","doi-asserted-by":"publisher","award":["TAY\u20102022\u201010040"],"award-info":[{"award-number":["TAY\u20102022\u201010040"]}],"id":[{"id":"10.13039\/501100004045","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Comput Chem"],"published-print":{"date-parts":[[2025,1,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The BCR\u2010ABL tyrosine kinase which is responsible for the pathogenesis of chronic myeloid leukemia (CML), has emerged as a promising therapeutic target. To address this issue, we employed a comprehensive computational approach integrating virtual screening, molecular dynamics (MD) simulations, and MM\u2010GBSA (Molecular Mechanics\/Generalized Born Surface Area) analysis to identify potential inhibitors and elucidate their binding mechanisms. Initially, virtual screening was conducted on 994 compounds from the ZINC database and, these compounds were docked against wildtype and T315I mutant ABL1 for the Type I and Type II ABL1 kinase inhibition mechanisms. In our molecular docking analysis for Type I inhibition, compound 911 demonstrated notable affinity towards the wildtype ABL1, with a binding energy of \u221214.91\u2009kcal\/mol, while compound 972 showed significant binding affinity towards the mutant ABL1, with a binding energy of \u221214.27\u2009kcal\/mol. In the Type II inhibition mechanism, the compounds with the highest binding affinity were compound 261 in wildtype ABL1 with \u221217.05\u2009kcal\/mol binding energy and compound 966 to the mutant ABL1 with a binding energy of \u221216.29\u2009kcal\/mol. Furthermore, analyses of MD simulations and MM\/GBSA binding free energy (\u0394G) were performed for target proteins with compounds, that exhibited the most favorable binding affinities with target proteins. The selected hit compounds showed \u0394G scores ranging from \u2212118.09 to \u221274.85\u2009kJ\/mol in both wildtype and mutant ABL1. Considering all in silico studies performed, it can be inferred that the identified molecules hold promise as potential candidates for drug design aimed at targeting CML.<\/jats:p>","DOI":"10.1002\/jcc.27545","type":"journal-article","created":{"date-parts":[[2024,12,5]],"date-time":"2024-12-05T10:10:31Z","timestamp":1733393431000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Exploring Inhibition Mechanisms in Wildtype and\n                    <scp>T315I BCR<\/scp>\n                    \u2010\n                    <scp>ABL1<\/scp>\n                    : An In Silico Approach Integrating Virtual Screening,\n                    <scp>MD<\/scp>\n                    Simulations, and\n                    <scp>MM<\/scp>\n                    \u2010\n                    <scp>GBSA<\/scp>\n                    Analysis"],"prefix":"10.1002","volume":"46","author":[{"given":"Ozlen","family":"Balta","sequence":"first","affiliation":[{"name":"Department of Hematology, Faculty of Medicine Karadeniz Technical University  Trabzon Turkey"},{"name":"Department of Bioinformatics, Institute of Health Sciences Karadeniz Technical University  Trabzon Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ercument","family":"Yilmaz","sequence":"additional","affiliation":[{"name":"Department of Computer Technologies Karadeniz Technical University  Trabzon Turkey"},{"name":"Y\u0131lmaz Bili\u015fim R&amp;D Consulting Software Engineering and Services Trade Limited Company  Trabzon Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6642-6870","authenticated-orcid":false,"given":"Gizem","family":"Tatar Yilmaz","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics, Institute of Health Sciences Karadeniz Technical University  Trabzon Turkey"},{"name":"Y\u0131lmaz Bili\u015fim R&amp;D Consulting Software Engineering and Services Trade Limited Company  Trabzon Turkey"},{"name":"Department of Biostatistics and Medical Informatics, Faculty of Medicine Karadeniz Technical University  Trabzon Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.2146\/ajhp070484"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40487\u2010019\u201000098\u2010w"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2022.102247"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029828"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.289.5486.1938"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M801337200"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2005.01.007"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747\u20100285.2010.01054.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1990"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010014\u20100461\u20108"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.2174\/156802610790232251"},{"key":"e_1_2_8_13_1","unstructured":"\u201cDiscovery Studio Visualizer v21. 1.0. 20298 (San Diego: Dassault Syst\u00e8mes 2020).\u201d"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/1758\u20102946\u20104\u201017"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1549"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.21256"},{"key":"e_1_2_8_17_1","volume-title":"Schr\u00f6dinger Release 2023\u20134 Desmond Molecular Dynamics System","author":"Schr\u00f6dinger L. L. C.","year":"2023"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.445869"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.470117"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3762\/bjoc.12.267"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022\u20102836(02)00470\u20109"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.10613"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2015.10.021"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201005\u20104187"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2014.07.006"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2011.03.003"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.13\u2010236992"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2018.08.011"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3MB70577C"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation\u20102008.1.436"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation\u20102007.1.371"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943\u2010018\u20100780\u20106"}],"container-title":["Journal of Computational Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcc.27545","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcc.27545","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcc.27545","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T03:49:25Z","timestamp":1787197765000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcc.27545"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,5]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1,5]]}},"alternative-id":["10.1002\/jcc.27545"],"URL":"https:\/\/doi.org\/10.1002\/jcc.27545","archive":["Portico"],"relation":{},"ISSN":["0192-8651","1096-987X"],"issn-type":[{"value":"0192-8651","type":"print"},{"value":"1096-987X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,5]]},"assertion":[{"value":"2024-07-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e27545"}}