{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,2]],"date-time":"2025-10-02T16:33:29Z","timestamp":1759422809524},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,3,15]],"date-time":"2002-03-15T00:00:00Z","timestamp":1016150400000},"content-version":"vor","delay-in-days":73,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The objective of this study was to analyze apparently discrepant results that arose during the use of an indirect immunofluorescence (IIF) assay using transfected HEp\u20102 cells to detect anti\u2010SS\u2010A\/Ro autoantibodies in human sera. Fourteen sera that had SS\u2010A\/Ro antibodies as detected on this commercial substrate, but did not have antibodies to SS\u2010A\/Ro as determined by double immunodiffusion (ID) or enzyme\u2010linked immunosorbent assay (ELISA), were studied by immunoprecipitation (IP) of radiolabeled cell extracts and full\u2010length recombinant SS\u2010A\/Ro. A multi\u2010antigen strip immunoblotting (IB) assay containing both the 52\u2010 and 60\u2010kDa antigens was included in the analysis. We confirmed that 12 of 14 of the sera under study had antibodies to SS\u2010A\/Ro protein antigens as determined by at least one other immunoassay. One serum had antibodies to hyRNA but no detectable reactivity with the 52\u2010 or 60\u2010kDa antigens. One serum remained negative in all assays for SS\u2010A\/Ro autoantibodies. J. Clin. Lab. Anal. 16:103\u2013108, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.10026","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T23:44:07Z","timestamp":1030319047000},"page":"103-108","source":"Crossref","is-referenced-by-count":29,"title":["Specificity of autoantibodies to SS\u2010A\/Ro on a transfected and overexpressed human 60 kDa Ro autoantigen substrate"],"prefix":"10.1002","volume":"16","author":[{"given":"Marvin J.","family":"Fritzler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheryl","family":"Hanson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joan","family":"Miller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Theophany","family":"Eystathioy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,3,15]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(61)92430-8"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.102.1.117"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI108007"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780220719"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6164096"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/32.5.396"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1016\/S0889-857X(21)00316-1","article-title":"Antinuclear antibodies in Sj\u00f6gren\u2019s syndrome","volume":"18","author":"Chan EKL","year":"1992","journal-title":"Rheum Dis Clin North Am"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00986765"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112892"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780270207"},{"key":"e_1_2_1_12_2","first-page":"101","article-title":"The particulate (speckled\u2010like thread) nuclear staining pattern: species and cellular distribution of the Ro\/SS\u2010A antigen","volume":"22","author":"Xia P","year":"1987","journal-title":"J Clin Lab Immunol"},{"key":"e_1_2_1_13_2","first-page":"67","article-title":"Detection of anti\u2010SS\u2010A\/Ro antibody by using pig spleen supernatant and characterization of corresponding antigen","volume":"2","author":"Takano S","year":"1989","journal-title":"Japan J Rheumatol"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.7.1996"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780281207"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.5.1560"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114014"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(90)90440-7"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1991.tb05780.x"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/32.6.449"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(90)90362-Y"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1258\/0004563001899032"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114551"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780340613"},{"key":"e_1_2_1_25_2","first-page":"1","volume-title":"Manual of biological markers of disease","author":"Chan EKL","year":"1994"},{"key":"e_1_2_1_26_2","first-page":"68","article-title":"A critical evaluation of enzyme immunoassay kits for the detection of antinuclear antibodies of defined specificities. 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