{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T06:10:54Z","timestamp":1698041454199},"reference-count":13,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,12,8]],"date-time":"2005-12-08T00:00:00Z","timestamp":1134000000000},"content-version":"vor","delay-in-days":6185,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[1989,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Examination of serum for the presence of antibodies to the human immunodeficiency virus (HIV) by immunoblot analysis requires precise identification of reactivities with various HIV specific proteins. During a recent survey of approximately 2,000 sera, we identified 22 sera from non\u2010HIV\u2010reactive blood donors and 2 from individuals receiving blood products for congenital blood disorders, which consistently and exclusively reacted with a protein of a molecular weight slightly greater than 65,000 daltons (termed AT65). Since the HIV <jats:italic>pol<\/jats:italic> p65 protein reacts with specific antibodies at about the same position (i.e., 65,000 daltons), it was essential to determine the viral or nonviral origin of the AT65 reactivity. Our data indicate that the AT65 reaction is due to a protein present on normal or activated lymphocytes, which can co\u2010purify with HIV preparations used for immunoblot analysis. Recognition of HIV\u2010specific p65 and nonspecific AT65 reactions is important to those responsible for interpretation of HIV immunoblots and may aid in the evaluation of some \u201cindeterminant\u201d results.<\/jats:p>","DOI":"10.1002\/jcla.1860030303","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T19:28:56Z","timestamp":1173122936000},"page":"148-151","source":"Crossref","is-referenced-by-count":2,"title":["Immunoblot reactivity of a non\u2010hiv protein with human sera"],"prefix":"10.1002","volume":"3","author":[{"given":"Gary L.","family":"Norman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu Su","family":"Barker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suraiya","family":"Rasheed","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"The Transfusion Safety Study Group","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,8]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1986.26286152918.x"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198604033141412"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1986.26186124012.x"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(86)90260-6"},{"key":"e_1_2_1_6_2","unstructured":"RasheedS NormanGL SuS:Acquired immune deficiency syndrome: Diagnostic procedures for the antibody and retrovirus. In Proc Int Symp Viral Hepatitis and AIDS Costa Rica pp31\u201352 1987."},{"key":"e_1_2_1_7_2","unstructured":"NormanGL WeissJN RasheedS: Variations in antibody reactivity to HIV detected by protein immunoblot and correlation with individual clinical history. Proc. Modern Approaches to New Vaccines including Prevention of AIDS Cold Spring Harbor p97 1986."},{"key":"e_1_2_1_8_2","unstructured":"NormanGL SuS.Gill PS LevineAM RasheedS: Absence of Antibodies to HIV core protein correlates with the development of symptomatic illness in patients at risk to develop AIDS. III Int Conf on AIDS. Washington DC p134 1987."},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6200935"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(85)90092-2"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(85)92910-1"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(85)92544-9"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00915045"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(85)90405-X"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.1860030303","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.1860030303","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T05:18:43Z","timestamp":1697951923000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.1860030303"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,1]]},"references-count":13,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1989,1]]}},"alternative-id":["10.1002\/jcla.1860030303"],"URL":"https:\/\/doi.org\/10.1002\/jcla.1860030303","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,1]]}}}