{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T01:59:21Z","timestamp":1744163961613},"reference-count":18,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,12,8]],"date-time":"2005-12-08T00:00:00Z","timestamp":1134000000000},"content-version":"vor","delay-in-days":6185,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[1989,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The <jats:italic>Wb<\/jats:italic> E34 monoclonal antibody raised against <jats:italic>Wuchereria bancrofti<\/jats:italic> microfilarial excretory secretory (mf ES) antigen was reported to be useful in detecting the filarial antigen in <jats:italic>W. bancrofti<\/jats:italic> and <jats:italic>Brugia malayi<\/jats:italic> infected sera. Further studies in this laboratory showed that this monoclonal antibody reacts with a stage\u2013specific antigen of <jats:italic>W. bancrofti<\/jats:italic> filarial parasite. <jats:italic>Wb<\/jats:italic> E34 identified three antigenic components with molecular weights, 55, 57.5, and 63 kilodaltons (Kd) in western blot analysis. The target antigen of <jats:italic>Wb<\/jats:italic> E34 was found to be located in the cytoplasm of microfilariae by indirect immunofluorescence technique. The inhibitory antibodies to <jats:italic>Wb<\/jats:italic> E34 were detected in a higher percentage of microfilaraemic sera (81%) than in clinical filarial (33%) or tropical eosinophilia sera (35%). Thus the inhibition ELISA using <jats:italic>W bancrofti<\/jats:italic> mf ES antigen and <jats:italic>Wb<\/jats:italic> E34\u2010penicil\u2010linase may be useful in detecting the filarial antibody associated with the active stage of infection.<\/jats:p>","DOI":"10.1002\/jcla.1860030504","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T19:49:49Z","timestamp":1173124189000},"page":"277-281","source":"Crossref","is-referenced-by-count":2,"title":["<i>Wb<\/i> e34 monoclonal antibody: Further characterization and diagnostic use in bancroftian filariasis"],"prefix":"10.1002","volume":"3","author":[{"given":"M. V. R.","family":"Reddy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. A.","family":"Parkhe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. 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