{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T17:03:44Z","timestamp":1769187824958,"version":"3.49.0"},"reference-count":19,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,12,12]],"date-time":"2005-12-12T00:00:00Z","timestamp":1134345600000},"content-version":"vor","delay-in-days":5824,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[1990,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The study reported here evaluated the usefulness of the enzyme\u2010linked immunosorbent assay (ELISA) in the detection of antibodies against pigeon antigens in the serum and bronchoalveolar lavage (BAL) of patients with clinical, radiological, and functional evidence of interstitial lung disease (ILD) with and without pigeon breeder's disease (PBD). The results were compared with those obtained by the simultaneous use of counterimmuno\u2010electrophoresis (CIE) in the same patients. In PBD, ELISA detected antibodies against pigeon's sera in both serum and BAL in 100% of patients, while CIE failed to detect the anti\u2010bodies in the serum of one patient and in most of the samples of BAL. In addition, we used enzyme immunotransfer blotting to determine the number of epitopes in pigeon serum recognized by antibodies present in serum and BAL. There was a heterogeneous response in both fluids, but the reaction pattern demonstrated that patients' sera recognize to\u201425 different pigeon epitopes. We conclude that ELISA is a highly sensitive and specific method for the detection of antibodies against pigeon antigens in the serum and BAL of patients with PBD and that the host response involves a great number of avian antigens.<\/jats:p>","DOI":"10.1002\/jcla.1860040202","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T19:46:17Z","timestamp":1173123977000},"page":"81-85","source":"Crossref","is-referenced-by-count":20,"title":["Detection of antibodies against avian antigens in bronchoalveolar lavage from patients with pigeon breeder's disease: Usefulness of enzyme\u2010linked immunosorbent assay and enzyme immunotransfer blotting"],"prefix":"10.1002","volume":"4","author":[{"given":"J.","family":"Sandoval","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. L.","family":"Ba\u00f1ales","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. J.","family":"Cort\u00e9s","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. A.","family":"Reyes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Mendoza","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Selman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02714434"},{"key":"e_1_2_1_3_2","first-page":"602","article-title":"Extrinsic allergic alveolitis caused by pigeon breeding at a high altitude (2 240 meters)","volume":"124","author":"Lupi HE","year":"1981","journal-title":"Am Rev Respir Dis"},{"key":"e_1_2_1_4_2","first-page":"561","article-title":"Neumonitis por hipersensibilidad a antigenos de paloma","volume":"54","author":"Reyes PA","year":"1984","journal-title":"Arch Inst Cardiol Mex"},{"key":"e_1_2_1_5_2","first-page":"147","article-title":"La funci\u00f3n respiratoria en la alveolitis al\u00e9rgica extrinseca por palomas","volume":"52","author":"Sandoval J","year":"1982","journal-title":"Arch Inst Cardiol Mex"},{"key":"e_1_2_1_6_2","first-page":"20","article-title":"Antibodies against pigeon serum proteins in pigeon breeders","volume":"71","author":"Fink JN","year":"1968","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_7_2","unstructured":"ReynoldsHY. Hypersensitivity pneumonitis.Clin Chest Med508\u2013518 1982."},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(76)90543-X"},{"key":"e_1_2_1_9_2","first-page":"698","volume-title":"Manual of Clinical Laboratory Immunology","author":"Fink JN","year":"1986"},{"key":"e_1_2_1_10_2","first-page":"97","article-title":"Neumonitis por hipersensibilidad a palomas. I. Valor clinico de la CIE para detectar anticuerpo en suero","volume":"51","author":"Reyes PA","year":"1981","journal-title":"Arch Inst Cardiol Mex"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(78)90184-7"},{"key":"e_1_2_1_12_2","first-page":"698","volume-title":"Manual of Clinical Laboratory Immunology","author":"Voller A","year":"1986"},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrilamide gels to nitrocellulose sheets: Procedure and applications","volume":"76","author":"Towbin H","year":"1979","journal-title":"Proc Natl Acad Sci USA"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1965.03090040005001"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.61.3.213"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.94.2.347"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.4049\/jimmunol.129.4.1493","article-title":"The antigens of pigeon breeder's disease. VII. Isoelectric cusing studies on unfractionated pigeon dropping extract","volume":"129","author":"McCormick D","year":"1982","journal-title":"J Immunol"},{"key":"e_1_2_1_18_2","doi-asserted-by":"crossref","first-page":"38","DOI":"10.4049\/jimmunol.111.1.38","article-title":"Immune response of the human respiratory tract","volume":"111","author":"Waldman RH","year":"1973","journal-title":"J Immunol"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-88-3-355"},{"key":"e_1_2_1_20_2","first-page":"540","article-title":"Immunologic studies in hypersensitivity pneumonitis. Quantitative precipitins and complement fixing antibodies in symptomatic and asymptomatic pigeon breeders","volume":"85","author":"Moore VL","year":"1975","journal-title":"J Lab Clin Med"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.1860040202","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.1860040202","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T10:00:11Z","timestamp":1697968811000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.1860040202"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,1]]},"references-count":19,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1990,1]]}},"alternative-id":["10.1002\/jcla.1860040202"],"URL":"https:\/\/doi.org\/10.1002\/jcla.1860040202","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,1]]}}}