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The bound antibodies are revealed using enzyme\u2010labeled antihuman IgG antibodies. Reliable results are obtained when the assay is carried out at 37\u00b0C. The reported data indicate that the dot\u2010ELISA can reliably be used for the detection of IgG antibodies to mumps and varicella viruseis in human sera.<\/jats:p>","DOI":"10.1002\/jcla.1860040303","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T20:00:34Z","timestamp":1173124834000},"page":"165-169","source":"Crossref","is-referenced-by-count":1,"title":["A dot\u2010elisa for the detection of igg antibodies to mumps and varicella viruses"],"prefix":"10.1002","volume":"4","author":[{"given":"Maria S.","family":"Leonardi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Domenica","family":"Gazzara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebastiana","family":"Zummo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pasquale","family":"Mastroeni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mich\u00e8le","family":"Fattal\u2010German","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Bizzini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(77)90410-7"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0019-2791(71)90454-X"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2795(77)90091-5"},{"key":"e_1_2_1_5_2","volume-title":"Handbook of Experimental Immunology","author":"Fuchs S","year":"1978"},{"issue":"7","key":"e_1_2_1_6_2","first-page":"43","article-title":"Ultrasensitive enzyme\u2010immunoassay","volume":"8","author":"Schuurs AH","year":"1978","journal-title":"Scand J Immunol"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-34-3-475"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1093\/clinchem\/26.6.741","article-title":"Variability in the adsorption properties of microtiter plates used as solid support in enzyme immunoassay","volume":"26","author":"Kricka LJ","year":"1980","journal-title":"Clin Chem"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(82)90677-7"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1978.tb03879.x"},{"key":"e_1_2_1_11_2","first-page":"1","volume-title":"Principles of Competitive Protein\u2010Binding Assays","author":"Yalow RS","year":"1971"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1128\/jcm.13.4.738-741.1981","article-title":"Investigation of enzyme immunoassay courses: Development of rapid assay systems","volume":"13","author":"Yolken RH","year":"1981","journal-title":"J Clin Microbiol"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(84)90045-4"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"e_1_2_1_15_2","unstructured":"LeonardiMS ZummoS MastroeniP:A multiple dot\u2010ELISA forToxoplasma gondii Rubella virus cytomegalovirus and herpesvirus 1 and 2. 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