{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T13:09:44Z","timestamp":1726405784266},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T00:00:00Z","timestamp":1134086400000},"content-version":"vor","delay-in-days":5821,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[1990,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have developed an indirect sandwich enzyme\u2010lined immunosorbent assay (ELISA) for the detection of antibodies to <jats:italic>Histoplasma<\/jats:italic> ribosomes and histoplasmin; we used this test for demonstration of these antibodies in sera from proven cases of histoplasmosis and other infections. Serum dilutions from five negative controls used in each experiment were normalized against 50 normal sera, and a factor of the mean absorbance was used to establish a positive reaction. Antiribosomal antibodies were detected in 97% of the known histoplasmosis patients with ELISA titers ranging from 1:100 to over 1:12,800. In contrast, antibodies to histoplasmin were detected in only 75% of these sera; titers ranged from 1:100 to 1:12,800. Cross\u2010reactions with sera from other fungal infections (blastomycosis, coccidioidomycosis, paracoccidioidomycosis, cryptococcosis, candidiasis, and aspergillosis) were seen in 46% of the cases with ribosomes and 37% with histoplasmin. Fifty percent of the sera from tuberculosis patients gave positive reactions with ribosomes and 29% with histoplasmin. These results warrant further studies on the significance of antibodies to ribosomes and histoplasmin in immunity to hlistoplasmosis.<\/jats:p>","DOI":"10.1002\/jcla.1860040311","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T20:00:34Z","timestamp":1173124834000},"page":"199-207","source":"Crossref","is-referenced-by-count":17,"title":["Evaluation of an elsa for the detection of anti\u2010histoplasma ribosomal and antihistoplasmin antibodies in histoplasmosis"],"prefix":"10.1002","volume":"4","author":[{"given":"Chander","family":"Raman","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy","family":"Khardori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ram P.","family":"Tewari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larry A.","family":"Von Behren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. Joseph","family":"Wheat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,9]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"95","article-title":"Immunology: Its value in diagnosing systemic fungal infections","volume":"3","author":"Kaufman L","year":"1977","journal-title":"Contrib Microbiol Immunol"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/00362177885380361"},{"key":"e_1_2_1_4_2","first-page":"265","article-title":"The diagnostic approach in histoplasmosis","volume":"5","author":"Wheat LJ","year":"1983","journal-title":"Immunol Allergy Proc"},{"key":"e_1_2_1_5_2","first-page":"564","volume-title":"Manual of Clinical Immunology","author":"Kaufman L","year":"1980"},{"key":"e_1_2_1_6_2","first-page":"441","article-title":"Antibodies in histoplasmosis detected by use of yeast and mycelial antigens in immunodiffusion and electroimmunodiffusion","volume":"120","author":"Sweet GH","year":"1979","journal-title":"Am Rev Respir Dis"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1128\/jcm.10.1.116-118.1979","article-title":"Detection of Candida antigen in sera of patients with candidiasis by an enzyme\u2010linked immunosorbent assay\u2010inhibition technique","volume":"10","author":"Segal E","year":"1979","journal-title":"J Clin Microbiol"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(82)90309-X"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1164\/arrd.1983.128.1.65"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198601093140205"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1128\/jcm.23.3.493-499.1986","article-title":"Evaluation of cross\u2010reactions in Histoplasma capsulatum serologic tests","volume":"23","author":"Wheat J","year":"1986","journal-title":"J Clin Microbiol"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/138.5.605"},{"key":"e_1_2_1_13_2","unstructured":"YamadaT KhardoriN McConnachieP TewariRP: Natural and antibody\u2010dependent cellular cytotoxicity of macrophges and lymphocytes for yeast cells of Histoplasma capsulatum. Annual Meeting of the American Society for Microbiology Atlanta GA 1982."},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.3314\/jjmm1960.24.179"},{"key":"e_1_2_1_15_2","first-page":"236","article-title":"Factors influencing the production of H and M antigens by Histoplasma capsulatum: Development and evaluation of a shake culture procedure","volume":"23","author":"Ehrhard HB","year":"1972","journal-title":"Infect Immunol"},{"key":"e_1_2_1_16_2","first-page":"250","article-title":"Factors influencing the production of H and M antigens by Histoplasma capsulatum: Effect of physical factors and composition of medium","volume":"23","author":"Ehrhard HB","year":"1972","journal-title":"Infect Immunol"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1128\/iai.36.3.1013-1018.1982","article-title":"Blastogenic responses of lymphocytes from mice immunized by sublethal infection with yeast cells of Histoplasma capsulatum","volume":"36","author":"Tewari RP","year":"1982","journal-title":"Infect Immunol"},{"key":"e_1_2_1_18_2","first-page":"218","volume-title":"Methods in Enzymology","author":"Garvey JS","year":"1981"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-103-25476"},{"key":"e_1_2_1_20_2","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Fohn phenol reagent","volume":"193","author":"Lowry OH","year":"1951","journal-title":"J Biol Chem"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(60)90049-7"},{"key":"e_1_2_1_22_2","first-page":"359","volume-title":"Manual of Clinical Immunology","author":"Voller A","year":"1980"},{"key":"e_1_2_1_23_2","first-page":"896","article-title":"Serologic diagnosis with respect to histoplasmosis, coccidioidomycosis and blastomycosis and the problem of cross reactions","volume":"42","author":"Campbell CC","year":"1953","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.2307\/4592678"},{"key":"e_1_2_1_25_2","doi-asserted-by":"crossref","first-page":"1033","DOI":"10.1128\/iai.9.6.1033-1044.1974","article-title":"Characterization and evaluation of a soluble antigen complex prepared from the yeast phase of Histoplasma capsulatum","volume":"9","author":"Reeves MV","year":"1974","journal-title":"Infect Immunol"},{"key":"e_1_2_1_26_2","doi-asserted-by":"crossref","first-page":"138","DOI":"10.1128\/am.28.1.138-144.1974","article-title":"Characterization of the precipitin bands detected in the immunodiffusion test or paracoccidioidomycosis","volume":"28","author":"Restrepo A","year":"1974","journal-title":"Appl Microbiol"},{"key":"e_1_2_1_27_2","doi-asserted-by":"crossref","first-page":"932","DOI":"10.1128\/iai.25.3.932-938.1979","article-title":"Cross\u2010reactivity between antigens of Coccidioides immitis, Histoplasma capsulatum and Blasromcyes dermatitidis in lymphocyte transformation assays","volume":"25","author":"Cox RA","year":"1979","journal-title":"Infect Immunol"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1080\/00362178385380451"},{"key":"e_1_2_1_29_2","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1128\/iai.23.2.479-485.1979","article-title":"Common antigens among systemic disease fungi analyzed by two\u2010dimensional immunoelectrophoresis","volume":"23","author":"Huppert M","year":"1979","journal-title":"Infect Immunol"},{"key":"e_1_2_1_30_2","first-page":"276","article-title":"Serologic effects of histoplasmin skin testing","volume":"83","author":"Nicholas WM","year":"1961","journal-title":"Am Rev Resp Dis"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(82)90209-5"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.1860040311","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.1860040311","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T10:05:05Z","timestamp":1697969105000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.1860040311"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,1]]},"references-count":30,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1990,1]]}},"alternative-id":["10.1002\/jcla.1860040311"],"URL":"https:\/\/doi.org\/10.1002\/jcla.1860040311","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"type":"print","value":"0887-8013"},{"type":"electronic","value":"1098-2825"}],"subject":[],"published":{"date-parts":[[1990,1]]}}}