{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T06:10:52Z","timestamp":1698041452948},"reference-count":13,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,10,20]],"date-time":"2005-10-20T00:00:00Z","timestamp":1129766400000},"content-version":"vor","delay-in-days":5406,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[1991,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A rapid, sensitive, and specific enzyme immunometric assay for cyclosporine in whole blood has been developed for the aca\u00ae analyzer, using a monoclonal antibody from Sandoz, Ltd. Between\u2010run CVs ranged from 6.5 to 7.6% for samples containing between 60 and 400 ng\/mL cyclosporine. Sensitivity was better than 25 ng\/mL in the assay, which has an effective upper range of greater than 600 ng\/mL. Two correlation studies compared cyclosporine values from the Du Pont method to those determined by HPLC procedures in three hospital laboratories. The results from a total of 120 whole blood samples with CsA between 20 and 800 ng\/mL showed excellent correlation between the methodologies. HPLC and Du Pont CsA values from 10 day serial studies also correlated well for samples from a kidney, kidney\u2010pancreas, heart, and two liver transplant patients. We conclude that the Du Pont CSA assay provides accurate and reproducible results in a convenient format in less than 30 minutes.<\/jats:p>","DOI":"10.1002\/jcla.1860050307","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T20:11:26Z","timestamp":1173125486000},"page":"187-192","source":"Crossref","is-referenced-by-count":2,"title":["Rapid and specific cyclosporine assay for the du pont aca\u00ae discrete clinical analyzer performed directly on whole blood"],"prefix":"10.1002","volume":"5","author":[{"given":"Joel B.","family":"Hansen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. Phillip","family":"Lau","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher J.","family":"Janes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Warren K.","family":"Miller","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald A.","family":"Wiebe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,10,20]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Cyclosporine: Therapeutic Use in Transplantation","author":"Kahan BD","year":"1988"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198912213212507"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1093\/clinchem\/33.7.1269","article-title":"Critical issues in cyclosporine monitoring: report of the task force on cyclosporine monitoring","volume":"33","author":"Shaw LM","year":"1987","journal-title":"Clin Chem"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"1299","DOI":"10.1093\/clinchem\/35.7.1299","article-title":"Advances in cyclosporine pharmacology, measurement and therapeutic monitoring","volume":"35","author":"Shaw LM","year":"1989","journal-title":"Clin Chem"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"1309","DOI":"10.1093\/clinchem\/35.7.1309","article-title":"The continuing need for quality assessment of cyclosporine measurement","volume":"35","author":"Johnston A","year":"1989","journal-title":"Clin Chem"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1093\/clinchem\/35.1.56","article-title":"Distribution of cyclosporin A metabolites among plasma and cells in whole blood: effect of temperature, hematocrit and metabolite concentration","volume":"35","author":"Lensmeyer GL","year":"1989","journal-title":"Clin Chem"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1093\/clinchem\/34.2.250","article-title":"Specific 3H\u2010radioimmunoassay with a monoclonal antibody for monitoring cyclosporine in blood","volume":"34","author":"Ball PE","year":"1988","journal-title":"Clin Chem"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jat\/10.1.28"},{"key":"e_1_2_1_10_2","first-page":"427","volume-title":"Methods in Clinical Chemistry","author":"Bowers LD","year":"1987"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1093\/clinchem\/31.2.196","article-title":"Improved liquid\u2010chromatographic determination of cyclosporine, with concomitant detection of a cell\u2010bound metabolite","volume":"31","author":"Lensmeyer GL","year":"1985","journal-title":"Clin Chem"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"1809","DOI":"10.1093\/clinchem\/30.11.1809","article-title":"An automated affinitycolumn\u2010mediated, enzyme\u2010linked immunometric assay for digoxin on the Du Pont aca\u00ae discrete clinical analyzer","volume":"30","author":"Leflar CC","year":"1984","journal-title":"Clin Chem"},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1093\/clinchem\/30.3.417","article-title":"A highly sensitive affinity coloumnmediated immunometric assay, as exemplified by digoxin","volume":"30","author":"Freytag JW","year":"1984","journal-title":"Clin Chem"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/clpt.1985.174"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.1860050307","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.1860050307","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T22:45:31Z","timestamp":1698014731000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.1860050307"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,1]]},"references-count":13,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1991,1]]}},"alternative-id":["10.1002\/jcla.1860050307"],"URL":"https:\/\/doi.org\/10.1002\/jcla.1860050307","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,1]]}}}