{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T22:01:05Z","timestamp":1698357665873},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,10,20]],"date-time":"2005-10-20T00:00:00Z","timestamp":1129766400000},"content-version":"vor","delay-in-days":5406,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[1991,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Fifty\u2010four pairs of cervical biopsies ranging from minimal dysplasia to severe dysplasia were studied for the presence of human papillomavirus DNA by in situ hybridization. Two assays were performed on each biopsy. A 16 hour hybridization was used in one assay, while a 40 hour hybridization was utilized in the second assay. Increasing the hybridization time to 40 hours did not significantly increase the detection rate of HPV compared to the rate found using the 16 hour hybridization. Also, no difference in the detection rate of HPV was found by using one biopsy of the pair over the other biopsy of the pair. However, the performance of a single in situ assay on only one biopsy from each patient significantly underestimated the true prevalence of HPV. A single assay only detected 21\/33 (64%) patients with HPV. Implications of multiple testing of all histologically abnormal biopsies is discussed in relation to prospective follow\u2010up studies determining the usefulness of HPV typing in patient management.<\/jats:p>","DOI":"10.1002\/jcla.1860050408","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T20:06:39Z","timestamp":1173125199000},"page":"268-274","source":"Crossref","is-referenced-by-count":2,"title":["Demonstration of human papillomavirus dna by nucleic acid in situ hybridization in paired histologically abnormal cervical biopsies obtained at the same patient visit"],"prefix":"10.1002","volume":"5","author":[{"given":"Antonio J.","family":"Amortegui","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael P.","family":"Meyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lara","family":"Kunschner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriana","family":"Saker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,10,20]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-8258(90)90327-H"},{"key":"e_1_2_1_3_2","first-page":"993","article-title":"Human papillomavirus types and localization in adenocarcinoma and adenosquamous carcinoma of the uterine cervix: a study by in\u2010situ DNA hybridization","volume":"48","author":"Tase T","year":"1988","journal-title":"Cancer Res"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1956.tb32134.x"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(68)90021-X"},{"key":"e_1_2_1_6_2","first-page":"307","article-title":"The biology and significance of human papillomavirus in the genital tract","volume":"61","author":"Reid R","year":"1988","journal-title":"Yale J Biol Med"},{"key":"e_1_2_1_7_2","first-page":"583","article-title":"Epidermodysplasia verruciformis as a model in studies on the role of papovaviruses in oncogenesis","volume":"32","author":"Jablonska S","year":"1972","journal-title":"Cancer Res"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(88)90093-2"},{"key":"e_1_2_1_9_2","first-page":"273","article-title":"Integration and persistence of human papillomavirus DNA in genital tumors","volume":"21","author":"Durst M","year":"1986","journal-title":"Bamberg Rep."},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0090-8258(88)90225-9"},{"key":"e_1_2_1_11_2","first-page":"781","article-title":"Human papillomavirus genotype as a prognostic indicator in carcinoma of the uterine cervix","volume":"74","author":"Walker J","year":"1989","journal-title":"Obstet Gynecol"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19881001)62:7<1331::AID-CNCR2820620715>3.0.CO;2-U"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004347-198903000-00002"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.2.2.166"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1990.tb01008.x"},{"key":"e_1_2_1_16_2","unstructured":"MeyerMP MarkiwCA MatuscakRR SakerA McIntyre\u2010SeltmanK AmorteguiAJDetection of human papillomavirus DNA in genital lesions using a modified commercially\u2010available in\u2010situ hybridization assay.J Clin Microbiol(publication\u2010in\u2010press)."},{"key":"e_1_2_1_17_2","first-page":"697","article-title":"Detection of conditions related to human papillomavirus. 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