{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T11:13:18Z","timestamp":1698145998474},"reference-count":17,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,10,20]],"date-time":"2005-10-20T00:00:00Z","timestamp":1129766400000},"content-version":"vor","delay-in-days":5041,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[1992,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The cellular nuclear antigen SS\u2010B\/La is known to be a major antigenic target to an autoantibody in patients with Sjogren's syndrome and systemic lupus erythematosus. It is useful to detect an anti\u2010SS\u2010B\/La antibody from patients' sera in a clinical point of view. We purified SS\u2010B\/La from rabbit thymus acetone powder by affinity chromatography with a murine anti\u2010SS\u2010B\/La monoclonal antibody (1C3\u2010H7). An enzyme\u2010linked immunosorbent assay method, in which SS\u2010B\/La was used to coat a plate, was also successfully established. It is difficult to obtain a large volume of patients' serum with high antibody titer and high specificity as a positive control. We investigated whether or not a positive control from human could be replaced by a murine monoclonal antibody to SS\u2010B\/La. The 1C3\u2010H7 was conjugated with a human IgG Fc' fragment using N\u2010\u03b3\u2010maleimidobutyryloxysuccinimide as a cross\u2010linker. The chemically humanized murine monoclonal antibody (1C3\u2010Fc') was recognized by antiserum specific for human IgG Fc fragment. 1C3\u2010Fc' reacted to SS\u2010B\/La but not to other antigens. Furthermore, the titration curve of this conjugate ran parallel with those of patients' sera specific for SS\u2010B\/La. It is concluded that a chemically humanized murine monoclonal antibody is useful as a positive control in place of a human patient's serum. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.1860060602","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T20:32:47Z","timestamp":1173126767000},"page":"343-350","source":"Crossref","is-referenced-by-count":4,"title":["Chemically humanized murine monoclonal antibody against a cell nuclear antigen: Usefulness in autoimmune diagnostics"],"prefix":"10.1002","volume":"6","author":[{"given":"Junko","family":"Miyachi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuyuki","family":"Doi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuyuki","family":"Kitamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomofumi","family":"Jitsukawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,10,20]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.22.6772"},{"key":"e_1_2_1_3_2","first-page":"1134","article-title":"La antigen recognizes and binds to the 3\u2032\u2010oligourydylate tail of a small RNA","volume":"4","author":"Matheus MB","year":"1984","journal-title":"Mol Celt Biol"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"8352","DOI":"10.1016\/S0021-9258(20)82072-9","article-title":"Identification of a La protein binding site in a RNA polymerase III transcrip (4.51 RNA)","volume":"258","author":"Reddy R","year":"1983","journal-title":"J Biol Chem"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(82)90099-X"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90083-7"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.11.5495"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"11441","DOI":"10.1016\/S0021-9258(17)44244-X","article-title":"Association between the 7S RNA and the lupus La protein varies among cell types","volume":"258","author":"Chambers JC","year":"1983","journal-title":"J Biol Chem"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb03445.x"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb03446.x"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/13.7.2617"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"1929","DOI":"10.1016\/S0021-9258(17)43496-X","article-title":"Eukaryotic small ribonucleoproteins. Anti\u2010La human autoantibodies react with U1 RNA\u2010protein complexes","volume":"259","author":"Madore SJ","year":"1984","journal-title":"J Biol Chem"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.19.5827"},{"issue":"10","key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"3744","DOI":"10.4049\/jimmunol.136.10.3744","article-title":"Epitopes, structural domains and asymmetry of amino acid residues in SS\u2010B\/La nuclear protein","volume":"136","author":"Chan EKL","year":"1986","journal-title":"J Immunol"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.7.7.2588"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0896-8411(89)90162-5"},{"key":"e_1_2_1_17_2","first-page":"932","article-title":"Purification of an acid nuclear protein antigen and demonstration of its antibodies in subsets of patients with sicca syndrome","volume":"119","author":"Akizuki M","year":"1977","journal-title":"J Clin Immunol"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(87)90020-6"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.1860060602","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.1860060602","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T17:22:03Z","timestamp":1698081723000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.1860060602"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,1]]},"references-count":17,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1992,1]]}},"alternative-id":["10.1002\/jcla.1860060602"],"URL":"https:\/\/doi.org\/10.1002\/jcla.1860060602","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,1]]}}}