{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T11:13:36Z","timestamp":1698146016253},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,10,20]],"date-time":"2005-10-20T00:00:00Z","timestamp":1129766400000},"content-version":"vor","delay-in-days":5041,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[1992,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We designed a rapid, homogeneous assay for human asparatate aminotransferase (AST) isoenzymes, by a selective proteolysis of soluble AST (s\u2010AST), using chymotryspin and protease 401. The linearity of mitochondrial (m\u2010AST) was elongated up to 4000 U\/l. m\u2010AST values from the human liver, and determined by a homogeneous assay using protease 401 or chymotryspin, were relative to those obtained using an immunoprecipitaiton method.<\/jats:p><jats:p>In perioperative patients or those with an acute myocardial infarction, the peaks of s\u2010AST and m\u2010AST values were noted 13 h and at 57 h after ictus, respectively, whereas the peak of ratio between was seen 6 h after ictus. In the case of Budd\u2010Chiari syndrome, the maximum levels of the two AST activities were evident 14 days after hospitalization and the peak of ratio between them was seen after 7 days. We propose that htis homogeneous assay can serve as a diagnostic tool for early phase detection of myocardial infarction and of Budd\u2010Chiari syndrome. \u00a9 1992 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.1860060605","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T20:32:47Z","timestamp":1173126767000},"page":"362-367","source":"Crossref","is-referenced-by-count":1,"title":["A homogeneous assay system of aspartate aminotransferase iso\u2010enzymes using proteases and application for clinical evaluation of myocardial infarction"],"prefix":"10.1002","volume":"6","author":[{"given":"Koji","family":"Yoneda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yosiaki","family":"Katayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiyo","family":"Koike","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isao","family":"Tanimizu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,10,20]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"441","article-title":"Comparative studies of glutamic oxaloacetic transaminases from the mitochondrial and soluble fractions of mammalian tissues","volume":"22","author":"Wada H","year":"1964","journal-title":"Vitamins and Hormones"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"8842","DOI":"10.1016\/S0021-9258(17)34254-0","article-title":"Cystosolic and mitochondrial isoenzymes of glutamic\u2010oxalacetic transminase from human heart","volume":"253","author":"Teranishi H","year":"1978","journal-title":"J Biol Chem"},{"key":"e_1_2_1_4_2","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/S0021-9258(17)43517-4","article-title":"Selective tryptic cleavage of native cytoplasmic aspartate transminase holoenzyme","volume":"259","author":"Iriarte A","year":"1984","journal-title":"J Biol Chem"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"1805","DOI":"10.1093\/clinchem\/24.10.1805","article-title":"Column chromatographic separation of aspartate aminotransferase","volume":"24","author":"Sampson EJ","year":"1978","journal-title":"Clin Chem Acta"},{"key":"e_1_2_1_6_2","first-page":"323","article-title":"On the determination of isoenzyme levels in preparations containing cytoplasmic and mitochondrial aspartate aminotransferase","volume":"482","author":"Martinez\u2010Caarrion M","year":"1977","journal-title":"Biophys Acta"},{"key":"e_1_2_1_7_2","first-page":"293","article-title":"Eine einfache kinetishe methode zum nachweis von zytoplasmatischer und mitochondrialer GOT ohne vorherige auftrennung","volume":"32","author":"Graubaum HJ","year":"1974","journal-title":"Acta Biol Med Chem"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1093\/clinchem\/27.4.535","article-title":"Measurement of aspartate aminotransferase isoenzymes. 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