{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T06:34:21Z","timestamp":1774593261712,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,10,20]],"date-time":"2005-10-20T00:00:00Z","timestamp":1129766400000},"content-version":"vor","delay-in-days":4675,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The vero cell lysate antigen for the enzymelinked immunosorbent assay (ELISA) of flaviviruses was evaluated for sensitivity, specificity including cross\u2010reactions, and background by comparing with the standard ELISA. Human sera, in serial dilutions, were taken from subjects 14, 35, and 210 days postvaccination with 17D antigen. Early after injection, high sensitivity (82.9%) was shown by the cell lysate antigen method. Late after infection, high sensitivity was achieved by the standard method (96.2% and 94%), with significant difference (<jats:italic>P<\/jats:italic> = 0.0001). However, sensitivity achieved by the cell lysate antigen method was also acceptable (91.7% &amp; 88.9%). The cell lysate antigen method showed high specificity and low cross reactivity early after infection. At 35 days postvaccination, no difference in specificity was observed between the two methods, but higher cross\u2010reactions were observed for the standard method. This pattern continued at 210 days postvaccination, with significantly higher cross\u2010reactions with the standard ELISA. The optical density differences by the two methods did not show significant relationship with the serial dilutions of human sera. No difference was observed in early and late infections in the background values of the negative control (Western equine encephalitis) between the two methods. The ELISA by the cell lysate antigen, within the limits of the experiments done, was found to be a good replacement for the ELISA by the standard method. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.1860070408","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T20:48:06Z","timestamp":1173127686000},"page":"230-237","source":"Crossref","is-referenced-by-count":33,"title":["Evaluation of vero cell lysate antigen for the ELISA of flaviviruses"],"prefix":"10.1002","volume":"7","author":[{"given":"Mohammad Z.","family":"Ansari","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert E.","family":"Shope","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sohail","family":"Malik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,10,20]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4613-3988-5_4"},{"key":"e_1_2_1_3_2","first-page":"1","article-title":"Dengue haemorrhagic fever\u2014A public health problem and afield for research","volume":"58","author":"Halstead SB","year":"1980","journal-title":"Bull WHO"},{"key":"e_1_2_1_4_2","first-page":"513","article-title":"Arthropod\u2010borne encephalitides in the Americas","volume":"57","author":"Monath TP","year":"1979","journal-title":"Bull WHO"},{"key":"e_1_2_1_5_2","first-page":"321","volume-title":"Maxcy\u2010Rosenau Public Health and Preventive Medicine","author":"Monath TP","year":"1980"},{"key":"e_1_2_1_6_2","first-page":"69","article-title":"Control and eradication of exotic viruses affecting man","volume":"26","author":"Murphy FA","year":"1979","journal-title":"Prog Med Virol"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-625380-1.50008-4"},{"key":"e_1_2_1_8_2","first-page":"2","volume-title":"The Arboviruses: Epidemiology and Ecology","author":"Monath TP","year":"1988"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0019-2791(71)90454-X"},{"key":"e_1_2_1_10_2","first-page":"1","volume-title":"The Enzyme Linked Immunosorbent Assay (ELISA); 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