{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,9]],"date-time":"2024-09-09T02:31:48Z","timestamp":1725849108226},"reference-count":14,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,10,20]],"date-time":"2005-10-20T00:00:00Z","timestamp":1129766400000},"content-version":"vor","delay-in-days":4675,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Diagnosis of von Willebrand's disease (vWD) requires quantitation of von Willebrand factor (vWF) in plasma plus qualitative assessment of the vWF multimers according to molecular size ranges. Characterization of vWF multimeric size distributions is typically done using sodium dodecyl sulfate\u2010agarose gel electrophoresis (SDS\u2010AGE) followed by immunoblotting in the gel with radiolabeled antibody against vWF and autoradiographic exposure. We applied a western blot technique to vWF multimeric analysis. It included SDS\u2010AGE, electroblotting onto a membrane, and chemiluminescent detection using rabbit anti\u2010human vWF as primary antibody and goat anti\u2010rabbit IgG as secondary antibody conjugated with horseradish peroxidase. Using this method, 18 to 20 vWF multimers were regularly resolved in normal plasma with exposure times of 2 to 4 sec compared to 4 hr or longer by autoradiography. Sensitivity of detection was at least 4\u2010fold enhanced by chemiluminescence compared to radiolabel. Specificity of the assay was confirmed by analysis of plasma samples known to be deficient to different degrees in the larger vWF multimers. The chemiluminographic assay for vWF multimers is superior to the autoradiographic one because it is more sensitive, avoids use of radioactivity, and has shorter total assay time (under 2 days versus five radiolabel). \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.1860070604","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T20:38:42Z","timestamp":1173127122000},"page":"317-323","source":"Crossref","is-referenced-by-count":2,"title":["Chemiluminographic detection of von Willebrand factor multimeric composition"],"prefix":"10.1002","volume":"7","author":[{"given":"Long T.","family":"Wen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo Marie","family":"Smolec","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Coughlin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard A.","family":"McPherson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,10,20]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V70.4.895.895"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.5.1906"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/newbio241217a0"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI112064"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109795"},{"key":"e_1_2_1_7_2","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.1182\/blood.V57.6.1140.1140","article-title":"The complete multimeric composition of factor VIII von Willebrand factor","volume":"57","author":"Ruggeri ZM","year":"1981","journal-title":"Blood"},{"key":"e_1_2_1_8_2","first-page":"481","volume-title":"Electrophoresis 84\u2014Proceedings of the Fourth Meeting of the International Electrophoresis Society, Gottingen","author":"Schneppenheim R","year":"1984"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1661533"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(85)90263-4"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(86)90078-2"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1055\/s-0038-1661530","article-title":"Electroblot and immunoperoxidase staining for rapid screening of the abnormalities of the multimeric structure of von Willebrand factor in von Willebrand's disease","volume":"55","author":"Lombardi R","year":"1986","journal-title":"Thromb Haemostas"},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1055\/s-0038-1642536","article-title":"Visualization of von Willebrand factor multimers by enzyme\u2010conjugated secondary antibodies","volume":"55","author":"Brosstad F","year":"1986","journal-title":"Thromb Haemostas"},{"key":"e_1_2_1_14_2","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1055\/s-0038-1647016","article-title":"Luminography\u2014an alternative assay for detection of von Willebrand factor multimers","volume":"60","author":"Schneppenheim H","year":"1988","journal-title":"Thromb Haemostas"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-0038-1645215"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.1860070604","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.1860070604","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T06:59:30Z","timestamp":1698217170000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.1860070604"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,1]]},"references-count":14,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1993,1]]}},"alternative-id":["10.1002\/jcla.1860070604"],"URL":"https:\/\/doi.org\/10.1002\/jcla.1860070604","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,1]]}}}