{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T05:47:24Z","timestamp":1761630444099},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,10,20]],"date-time":"2005-10-20T00:00:00Z","timestamp":1129766400000},"content-version":"vor","delay-in-days":4310,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Allotransplant rejection is a T\u2010cell\u2010dependent reaction. Functional in vitro T\u2010cell assays are being used widely for donorrecipient matching in bone marrow transplantation and have recently been used in some centres for transplant monitoring. In order to assess tolerance induction after clinical transplantation, we measured the T\u2010cell response of the host against donor spleen cells of 33 kidney transplant patients before and every 3 months after transplantation over a period of 18 months. The T\u2010cell reactivity before transplantation was not significantly different in any of the assays in rejecting and nonrejecting patients. In the classical mixed lymphocyte culture (MLC), a donor\u2010specific loss of reactivity was seen only in a patient with a CMV\u2010associated irreversible transplant rejection. One patient with chronic rejection acquired a very high MLC response against donor spleen cells and a high response against third\u2010party cells. Little or nonspecific changes were seen in the MLCs of all other patients. Using the method of limiting dilution analysis (LDA), we found a significant reduction of donor\u2010specific cytotoxic T\u2010cell precursors (CTL\u2010p) within the first 3 months after transplantation in most patients with high antidonor CTL\u2010p frequencies before transplantation. The reduction of donor\u2010specific CTL\u2010p was seen in patients with rejection episodes as well as in patients without. Thus we conclude, in contrast to others, that MLC and CTL\u2010p LDA have no predictive value on the outcome of clinical transplantation. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.1860080308","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T20:43:06Z","timestamp":1173127386000},"page":"157-162","source":"Crossref","is-referenced-by-count":26,"title":["Failure of in vitro T\u2010cell assays to predict clinical outcome after human kidney transplantation"],"prefix":"10.1002","volume":"8","author":[{"given":"J.","family":"Steinmann","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Kaden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"May","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Schr\u00f6der","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Herwartz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"M\u00fcller\u2010Ruchholtz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,10,20]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"630","article-title":"Failure of in vitro assays to predict accurately the existence of neonatally induced H\u20102 tolerance","volume":"48","author":"Streilein JW","year":"1989","journal-title":"Transplantation"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-198103000-00014"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-198910000-00004"},{"key":"e_1_2_1_5_2","first-page":"1083","article-title":"Renal transplant patients with steroid withdrawal evaluated longitudinal for their donor\u2010specific cytotoxic T cell reactivity","volume":"52","author":"Goulmy E","year":"1992","journal-title":"Transplantation"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-198703000-00013"},{"key":"e_1_2_1_7_2","unstructured":"ZankerB Joo\u00df\u2010R\u00fcdigerJ FranzH\u2010E WagnerH KabelitzD: Functional deletion of donor\u2010reactive T\u2010lymphocytes in kidney allograft recipients can occur at the level of cytotoxic T\u2010cells IL\u20102 producing T\u2010cells or both: A limiting dilution study.Transplantation(in press)."},{"key":"e_1_2_1_8_2","first-page":"1873","article-title":"Clinical relevance of cytotoxic T cell precursor frequencies in human allograft recipients","volume":"22","author":"Steinmann J","year":"1990","journal-title":"Transpl Proc"},{"issue":"1","key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"684","DOI":"10.1111\/tri.1992.5.s1.684","article-title":"Rejection prophylaxis with interleukin\u20102 receptor antibody BT563: mechanisms of action on human cells","volume":"5","author":"Herwartz C","year":"1992","journal-title":"Transpl Int"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(90)90113-N"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0008-8749(91)90059-K"},{"key":"e_1_2_1_12_2","doi-asserted-by":"crossref","first-page":"1614","DOI":"10.4049\/jimmunol.126.4.1614","article-title":"Limiting dilution analysis for the determination of immunocompetent cell frequencies\nI. 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