{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,2,23]],"date-time":"2024-02-23T09:39:09Z","timestamp":1708681149186},"reference-count":23,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2005,10,20]],"date-time":"2005-10-20T00:00:00Z","timestamp":1129766400000},"content-version":"vor","delay-in-days":4310,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The diagnosis of Helicobacter pylori (Hp) infection is an important goal in clinical practice. In this paper we evaluated 1. the analytical reliability of a new second\u2010generation antigen based enzyme immunoassay (Cobas Core Anti Helicobacter pylori EIA) in detecting anti\u2010Hp IgG antibodies, and 2. the behaviour of anti\u2010Hp IgG in patients with chronic atrophic and non\u2010atrophic gastritis as compared to healthy controls. The findings from the dilution curve, the values of intra and inter assay coefficients of variations (never above 10%) and of the recovery test (between 96 and 109%), confirm that the method is reliable. Serum IgG anti\u2010Hp levels were found to be significantly higher in patients with histologically identified Hp infection, than in those negative at histology. Furthermore, the grade of histological positivity was correlated with serum IgG levels. However, we found a discrepancy between a low prevalence of Hp staining and a high prevalence of Hp seropositivity in patients with chronic atrophic or non\u2010atrophic gastritis, but not in controls. This suggests that IgG serum determination may be more useful than histology in determining a present or previous infection in patients with chronic atrophic or non\u2010atrophic gastritis. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.1860080407","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T21:49:27Z","timestamp":1173131367000},"page":"219-222","source":"Crossref","is-referenced-by-count":2,"title":["A new method for detecting anti Helicobacter pylori antibodies: An analytical and clinical evaluation"],"prefix":"10.1002","volume":"8","author":[{"given":"M.","family":"Plebani","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Basso","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Brigato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Cassaro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Farinati","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Di Mario","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Rugge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,10,20]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.293.6550.772"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199003293221307"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01298869"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199104113241507"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(88)90606-3"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/161.4.626"},{"key":"e_1_2_1_8_2","first-page":"285","article-title":"A topographical relationship between Helicobacter pylori and gastritis: quantitative assessment of Helicobacter pylori in the gastric mucosa","volume":"86","author":"Satoh K","year":"1991","journal-title":"Am J Gastroenterol"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01300747"},{"key":"e_1_2_1_10_2","first-page":"133","article-title":"Usefulness of culture, histology, and urease testing in the detection of Campylobacter pylori","volume":"84","author":"Schnell GA","year":"1989","journal-title":"Am J Gastroenterol"},{"key":"e_1_2_1_11_2","first-page":"26b","article-title":"Histologic demonstration of Campylobacter pylori","volume":"13","author":"De Mascarel A","year":"1989","journal-title":"Gastroenterol Clin Biol"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/12.Supplement_1.S107"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.30.6.798"},{"key":"e_1_2_1_14_2","first-page":"190","volume-title":"Campylobacter Pylori and Gastroduodenal Disease","author":"Newell DG","year":"1989"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.44.4.326"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)90085-4"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(91)90462-T"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(89)92761-X"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)90929-W"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01961663"},{"key":"e_1_2_1_21_2","first-page":"9","article-title":"Usefulness of two different assays for serodiagnosis of Helicobacter pylori infection","volume":"1","author":"Plebani M","year":"1993","journal-title":"Eur J Lab Med"},{"key":"e_1_2_1_22_2","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1128\/jcm.30.1.176-180.1992","article-title":"Evaluation of a commercially available second\u2010generation immunoglobulin G enzyme immunoassay for detection of Helicobacter pylori infection","volume":"30","author":"Goossens H","year":"1992","journal-title":"J Clin Microbiol"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(91)90474-Y"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.1993.tb00062.x"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.1860080407","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.1860080407","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T09:24:24Z","timestamp":1698225864000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.1860080407"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,1]]},"references-count":23,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1994,1]]}},"alternative-id":["10.1002\/jcla.1860080407"],"URL":"https:\/\/doi.org\/10.1002\/jcla.1860080407","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,1]]}}}