{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T05:53:26Z","timestamp":1698213206454},"reference-count":9,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,10,20]],"date-time":"2005-10-20T00:00:00Z","timestamp":1129766400000},"content-version":"vor","delay-in-days":4310,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An automated measurement of total and free hydroxyproline in serum or urine is presented that uses flow injection analysis. After exclusion of nonspecific substances, hydroxyproline was oxidized by chloramine\u2010 T and L\u2010cysteine with Ehrlich's reagent.<\/jats:p><jats:p>The linearity obtained was from 3.8\u03bcmole\/ L to 1.22 mmole\/L with good precision (CV &lt;3%). Comparison of the proposed method with HPLC yielded r = 0.939 as the correlation coefficient.<\/jats:p><jats:p>Reference intervals of free and total hydroxyproline are 1.4\u20139.7 \u03bcmole\/L, 3.8\u201327.2 \u03bcmole\/L for serum, and 10.0\u201372.5 \u03bcmole\/L, 25.2\u2013303.6 \u03bcmole\/L for urine, respectively. Serum free and total hydroxyproline levels in renal osteodystrophy patients on maintenance hemodialysis (N = 71) were significantly higher than in controls (<jats:italic>P<\/jats:italic>&lt;0.0001). This method is superior to the use of HPLC with regard to stability of the color reaction. The measurement of serum free and total hydroxyproline is a useful marker for therapeutic observation of renal osteodystrophy patients. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.1860080504","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T22:08:57Z","timestamp":1173132537000},"page":"267-272","source":"Crossref","is-referenced-by-count":5,"title":["Measurement of free and total hydroxyproline by automated flow injection of serum or urine samples from maintenance hemodialysis patients with renal osteodystrophy"],"prefix":"10.1002","volume":"8","author":[{"given":"Yoshinori","family":"Uji","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arthur","family":"Karmen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Okabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keishi","family":"Hata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masakazu","family":"Miura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuyuki","family":"Ozaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitsuo","family":"Minamizaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsushi","family":"Shibata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiichi","family":"Inayama","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,10,20]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/205264a0"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(66)90249-1"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(71)90438-6"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.2302\/kjm.27.43"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(79)90061-5"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.34.2649"},{"key":"e_1_2_1_8_2","first-page":"111","article-title":"A new rapid and convenient method for microdetermination of hydroxyproline using flow injection analysis","volume":"5","author":"Uchida K","year":"1988","journal-title":"J Flow Inject Anal"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.2116\/analsci.6.39"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(81)90390-0"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.1860080504","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.1860080504","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T02:20:55Z","timestamp":1698114055000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.1860080504"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,1]]},"references-count":9,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1994,1]]}},"alternative-id":["10.1002\/jcla.1860080504"],"URL":"https:\/\/doi.org\/10.1002\/jcla.1860080504","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,1]]}}}