{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T21:49:55Z","timestamp":1761947395448},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,10,20]],"date-time":"2005-10-20T00:00:00Z","timestamp":1129766400000},"content-version":"vor","delay-in-days":4310,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Antibodies directed against soluble liver antigen (SLA), liver kidney microsomal antigen (LKM\u20101\u2010AG), and antimitochondrial antigen M2 (M2\u2010AMA) are critical serological markers for the differential diagnosis of autoimmune chronic active hepatitis (AI\u2010CAH) and primary biliary cirrhosis (PBC). The exact diagnosis of autoimmune hepatitis and PBC is of great clinical relevance, as it leads to different therapeutic strategies.<\/jats:p><jats:p>In the present work, a simple and reliable ELISA test system is described, which applies the same test principle for the detection of three different species of autoantibodies important for the diagnosis of chronic liver disease. The ELISA assays are based on a competitive inhibition of binding of positive standard antibodies by patients sera containing antibodies of unknown specificity. The purified immunoglobulins of clinically and serologically clearly defined patients with SLA or LKM\u20101 positive AI\u2010CAH and with M2 positive PBC were used as coating\u2010 and detection antibodies in the ELISAs. From homogenized rat liver the fractionated 100,000g supernatant was employed for the SLA ELISA, the microsomal preparation served as antigen for the LKM\u20101 ELISA and the mitochondrial preparation was used for the M2 ELISA. In 1,500 sera of patients with the differential diagnosis of a hepatobiliary disease, 17 gave a positive signal in the SLA ELISA, 12 in the LKM\u20101\u2010ELISA, and 72 in the M2\u2010ELISA. The results of the ELISAs were compared with Western blotting and immunofluorescence staining pattern on cryostat sections and Hep2 cells. The antibody profiles of several patients are described in detail. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.1860080507","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T22:08:57Z","timestamp":1173132537000},"page":"284-292","source":"Crossref","is-referenced-by-count":6,"title":["Detection of autoantibodies against M2, LKM\u20101, and SLA in liver diseases by standardized uniform ELISA\u2010techniques"],"prefix":"10.1002","volume":"8","author":[{"given":"R.","family":"Gruber","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Felber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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