{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:43:45Z","timestamp":1761155025637},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,10,20]],"date-time":"2005-10-20T00:00:00Z","timestamp":1129766400000},"content-version":"vor","delay-in-days":4310,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[1994,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Quantitative chemiluminescent enzymelinked immunosorbent assay (ELISA) and dot\u2010blotting procedures were developed to evaluate the reactivity of human antibodies with crude antigens and purified molecules of parasites and fungi, mainly <jats:italic>Trypanosoma cruzi<\/jats:italic> and <jats:italic>Paracoccidioides brasiliensis.<\/jats:italic> Reproducible, highly sensitive, and strictly doseresponding results were obtained, with the specificity depending on the kind of antigen used. Mixed antigens (epimastigote membrane and HIV\u20101 heptapeptide) applied in dots could be independently recognized by specific sera. Purified antigens (<jats:italic>T. cruzi<\/jats:italic> F2\/3 and <jats:italic>P. brasiliensis<\/jats:italic> gp43) at very small concentrations gave specific reactions with patients' sera diluted \u22651:1,000 and were very poorly reactive or unreactive with natural antibodies using the chemiluminescent immunoassays. <jats:italic>P. brasiliensis<\/jats:italic> crude antigen Fava Netto polysaccharide antigen (FNPA) contained peptide epitopes recognized by natural antibodies and carbohydrate epitopes reactive with sera from histoplasmosis patients. It is very important that sensitive chemiluminescence immunoassays be used with purified antigenic molecules to ensure specificity for the diagnosis and follow\u2010up of parasitic and fungal infections. \u00a9 1994 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.1860080614","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T22:08:55Z","timestamp":1173132535000},"page":"424-431","source":"Crossref","is-referenced-by-count":16,"title":["Chemiluminescent immunoassays: Discrimination between the reactivities of natural and human patient antibodies with antigens from eukaryotic pathogens, <i>Trypanosoma cruzi<\/i> and <i>Paracoccidioides brasiliensis<\/i>"],"prefix":"10.1002","volume":"8","author":[{"given":"Igor C.","family":"Almeida","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elaine G.","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz R.","family":"Travassos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,10,20]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(92)90365-E"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.1860070604"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.6.2563"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.12.4514"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.1860050206"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109876"},{"key":"e_1_2_1_8_2","first-page":"280","article-title":"Stimulation of neutrophil chemiluminescence by soluble immune complexes and antibodies to netutrophils","volume":"98","author":"Starkebaum G","year":"1981","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_9_2","first-page":"905","article-title":"Luminescence immunoassays: Problems and possibilities","volume":"22","author":"Wood WG","year":"1984","journal-title":"J Clin Chem Clin Biochem"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(92)90165-4"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.1860070603"},{"key":"e_1_2_1_12_2","first-page":"69","article-title":"Antibody specificity in the conventional serology for Chagas disease","volume":"45","author":"Travassos LR","year":"1993","journal-title":"Ci Cult J Braz Assoc Adv Sci"},{"key":"e_1_2_1_13_2","doi-asserted-by":"crossref","first-page":"1610","DOI":"10.1128\/jcm.29.8.1610-1615.1991","article-title":"43 Kilodalton glycoprotein from Paracoccidioides brasiliensis: Immunochemical reactions with sera from patients with paracoccidioidomycosis, histoplamosis or Jorge Lobo's disease","volume":"29","author":"Puccia R","year":"1991","journal-title":"J Clin Microbiol"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1080\/02681219480000111"},{"key":"e_1_2_1_15_2","first-page":"93","article-title":"Growth and differentiation in Trypanosoma cruzi. 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