{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,21]],"date-time":"2025-06-21T04:10:41Z","timestamp":1750479041535},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,12,9]],"date-time":"2005-12-09T00:00:00Z","timestamp":1134086400000},"content-version":"vor","delay-in-days":3995,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[1995,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We improved our previously devised enzyme immunoassay (FIA) system for basic fibroblast growth factor (bFGF) using biotinylated antibody prepared with sulfosuccinimidyl\u20106\u2010(biotinamido)hexanoate (NHS\u2010LC\u2010Biotin, Pierce), a water\u2010soluble biotin analogue, with an extended spacer arm. The discriminatory detection limit of the improved EIA was found to be 5 pg\/ml (0.5 pg\/assay tube), sixfold more sensitive than that of the previous system. The reproducibility of within\u2010 and between\u2010assay series was 5.10\u20138.61% and 5.99\u20138.69%, respectively; and recovery of exogenous bFGF from serum was approximately 102%. Employing the improved EIA system, we investigated by chromatofocusing chromatography the isoelectric points of two immunoreactive bFGFs (high\u2010molecular\u2010weight bFGF\u2010like immunoreactive substance, designated as HMW\u2010bFGF\u2010LI and 16\u2010kd bFGF\u2010LI, having the same molecular weight as recombinant bFGF) detected in serum from a breast cancer patient. As a result, the pl value of HMW\u2010bFGF\u2010LI was estimated to be 7.13, and that of 16 kd bFGF\u2010LI, 9.58.\u00a91995 wiley\u2010Liss, inc.<\/jats:p>","DOI":"10.1002\/jcla.1860090204","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T22:04:23Z","timestamp":1173132263000},"page":"96-100","source":"Crossref","is-referenced-by-count":3,"title":["Improvement of an eia system for basic fibroblast growth factor by use of biotinylated antibody prepared with nhs\u2010lc\u2010biotin"],"prefix":"10.1002","volume":"9","author":[{"given":"Yoshifumi","family":"Takei","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanae","family":"Higashira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyozo","family":"Hayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,9]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.19.6507"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-8-2-95"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2544996"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.11.4378"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.4.1095"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3523756"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/325257a0"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc00020a005"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.3164\/jcbn.15.57"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/37.5.625"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1089\/hyb.1989.8.209"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(87)90553-5"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(88)90226-4"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1250781"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90123-0"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.3164\/jcbn.17.19"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.1860090204","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.1860090204","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T18:57:13Z","timestamp":1698346633000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.1860090204"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,1]]},"references-count":16,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1995,1]]}},"alternative-id":["10.1002\/jcla.1860090204"],"URL":"https:\/\/doi.org\/10.1002\/jcla.1860090204","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,1]]}}}