{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T14:10:53Z","timestamp":1698329453109},"reference-count":46,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,12,11]],"date-time":"2005-12-11T00:00:00Z","timestamp":1134259200000},"content-version":"vor","delay-in-days":3997,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[1995,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Sickle cell disease covers a group of conditions in which pathology may be attributed to the presence of sickle hemoglobin (HbS). The identification of HbS and other variants including those in combination with HbS is commonly achieved by cellulose acetate electrophoresis at alkaline pH. Because many hemoglobin variants with similar charges have similar electrophoretic migration patterns, they are difficult to differentiate by electrophoresis. The HemoCard<jats:sup>TM<\/jats:sup> assays address this concern through the use of monoclonal antibodies capable of specifically recognizing the unique amino acid sub\u2010stitution in the variant hemoglobin. The panel of HemoCard monoclonal antibodies confirms the absence and presence of HbA, HbC, HbE, HbS, and other sickling hemoglobin variants. The combination of alkaline cellulose acetate electrophoresis and HemoCard assays allows the technologist to reach a final confirmation of both common and much less common sickle cell disease genotypes, combinations of HbS with other hemoglobins that ordinarily do not produce sickle cell disease, and other clinically important hemoglobinopathies including HbE\/\u00df\u2010thalassemia and hemoglobin C disease.<\/jats:p>","DOI":"10.1002\/jcla.1860090606","type":"journal-article","created":{"date-parts":[[2007,3,5]],"date-time":"2007-03-05T22:10:32Z","timestamp":1173132632000},"page":"366-374","source":"Crossref","is-referenced-by-count":1,"title":["Utilization of monoclonal\u2010antibody\u2010based assay (hemocard<sup>tm<\/sup>) in screening for and differentiating between genotypes of sickle cell disease and other hemoglobinopathies"],"prefix":"10.1002","volume":"9","author":[{"given":"John C.","family":"Schultz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,11]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1093\/labmed\/21.12.797","article-title":"The sickling disorders","volume":"21","author":"Hoffman GC","year":"1990","journal-title":"Lab Med"},{"key":"e_1_2_1_3_2","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1093\/labmed\/21.12.815","article-title":"Neonatal hemoglobinopathy screening","volume":"21","author":"Armbruster DA","year":"1990","journal-title":"Lab Med"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/labmed\/24.8.515"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2257.1988.tb01156.x"},{"key":"e_1_2_1_6_2","volume-title":"Sickle Cell Disease","author":"Serjeant GR","year":"1992"},{"key":"e_1_2_1_7_2","first-page":"41","volume-title":"Surgery Annual","author":"Heller P","year":"1974"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-7091-8798-2_6"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.1093\/clinchem\/20.9.1111","article-title":"Differentiation of electrophoretically similar hemoglobins\u2014such as S, D, G, and P; or A2, C, E, and O\u2014by electrophoresis of the globin chains","volume":"20","author":"Schneider RG","year":"1974","journal-title":"Clin Chem"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1542\/peds.83.5.855","article-title":"Alternative methods for screening","volume":"83","author":"Garrick MD","year":"1989","journal-title":"Pediatrics"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.3109\/03630268408991704"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00281061"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/324163a0"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.867032"},{"key":"e_1_2_1_15_2","first-page":"167","article-title":"Immunochemical identification and quantitation of variant hemoglobins","volume":"40","author":"Garver FA","year":"1981","journal-title":"Texas Reports Biol Med"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.3109\/03630268508996995"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.2830380311"},{"issue":"1","key":"e_1_2_1_18_2","first-page":"141a","article-title":"Immunoassay for hereditary hemoglobin H disease","volume":"74","author":"Kiefer CR","year":"1989","journal-title":"Blood"},{"key":"e_1_2_1_19_2","first-page":"950","article-title":"Monoclonal immunoassay for identification of hemoglobin variants","volume":"37","author":"Rosenthal MA","year":"1991","journal-title":"Clin Chem"},{"key":"e_1_2_1_20_2","first-page":"1096","article-title":"Monoclonal antibody immunoassay for the identification of hemoglobin C","volume":"38","author":"Rosenthal MA","year":"1992","journal-title":"Clin Chem"},{"key":"e_1_2_1_21_2","unstructured":"Gelman Sciences: Hemoglobin electrophoresis system\u2014Product No. 51126 Ann Arbor MI."},{"key":"e_1_2_1_22_2","volume-title":"Detection of Abnormal Hemoglobin Using Cellulose Acetate Electrophoresis","author":"National Committee for Clinical Laboratory Standards","year":"1994"},{"key":"e_1_2_1_23_2","first-page":"279","volume-title":"Hemoglobinopathies and Thalassemias: Laboratory Methods and Clinical Cases","author":"Fairbanks VF","year":"1980"},{"key":"e_1_2_1_24_2","unstructured":"Isolab: Drawer 4350 Akron OH HemoCard Hemoglobin A and S: Monoclonal antibody based assay for determining the heterozygosity or homozygosity of hemoglobin S (HbS). 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