{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,21]],"date-time":"2025-11-21T22:51:38Z","timestamp":1763765498146},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,11,12]],"date-time":"2004-11-12T00:00:00Z","timestamp":1100217600000},"content-version":"vor","delay-in-days":316,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Bcl<\/jats:italic>\u20102 and survivin are cellular proteins that are known to be inhibitors of apoptosis and are commonly found in malignant tissues, including lymphomas. In previous studies, it has been shown that staining for <jats:italic>bcl<\/jats:italic>\u20102 can help distinguish between benign and malignant lymphoid aggregates in bone marrow biopsies. To determine whether staining for survivin expression in lymphoid aggregates can aid investigators in making this clinically important distinction, we stained bone marrow biopsies from 10 patients with benign lymphoid aggregates, and 15 malignant ones derived from B cells (six mantle cell, four follicular cells, two diffuse large cell, two small lymphocytic cell, and one marginal zone lymphoma) with antibodies to CD3, CD20, <jats:italic>bcl<\/jats:italic>\u20102, and survivin by an indirect immunoperoxidase technique. Whereas staining for <jats:italic>bcl<\/jats:italic>\u20102 was significantly stronger in the malignant lymphoid aggregates (<jats:italic>P<\/jats:italic>=0.001), both the control and malignant cases were almost uniformly negative for survivin expression. Only three cases (two mantle cell and one small lymphocytic lymphoma) showed very faint expression of survivin. Although <jats:italic>bcl<\/jats:italic>\u20102 and survivin both act to inhibit apoptosis, their expressions do not parallel each other. Survivin is not significantly expressed in either benign or malignant bone marrow aggregates, and therefore measuring its expression does not help distinguish benign from malignant B\u2010cell bone marrow lymphoid aggregates. J. Clin. Lab. Anal. 18:285\u2013288, 2004. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.20039","type":"journal-article","created":{"date-parts":[[2004,11,12]],"date-time":"2004-11-12T22:53:08Z","timestamp":1100299988000},"page":"285-288","source":"Crossref","is-referenced-by-count":9,"title":["Do <i>bcl<\/i>\u20102 and survivin help distinguish benign from malignant B\u2010cell lymphoid aggregates in bone marrow biopsies?"],"prefix":"10.1002","volume":"18","author":[{"given":"Amish M.","family":"Gandhi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jonathan M.","family":"Ben\u2010Ezra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,11,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5381.1322"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V88.3.1046.1046"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1994.22"},{"key":"e_1_2_1_5_2","first-page":"5071","article-title":"Inhibition of apoptosis by survivin predicts shorter survival rates in colorectal cancer","volume":"58","author":"Kawasaki H","year":"1998","journal-title":"Cancer Res"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0897-917"},{"key":"e_1_2_1_7_2","first-page":"560","article-title":"Staining for Bcl\u20102 protein helps to distinguish benign from malignant lymphoid aggregates in bone marrow biopsies","volume":"7","author":"Ben\u2010Ezra JM","year":"1994","journal-title":"Mod Pathol"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.9.2777"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.275.5303.1132"},{"key":"e_1_2_1_10_2","first-page":"5315","article-title":"IAP\u2010family protein survivin inhibits caspase activity and apoptosis induced by Fas (CD95), Bax, caspases, and anticancer drugs","volume":"58","author":"Tamm I","year":"1998","journal-title":"Cancer Res"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/25141"},{"key":"e_1_2_1_12_2","first-page":"1234","article-title":"Induction of apoptosis by bexarotene in cutaneous T\u2010cell lymphoma cells: relevance to mechanism of therapeutic action","volume":"8","author":"Zhang C","year":"2002","journal-title":"Clin Cancer Res"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/93.20.1541"},{"key":"e_1_2_1_14_2","first-page":"1921","article-title":"Prognostic significance of survivin expression in diffuse large B\u2010cell lymphomas","volume":"96","author":"Adida C","year":"2000","journal-title":"Blood"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402427"},{"key":"e_1_2_1_16_2","first-page":"595","article-title":"Quantitative analysis of the anti\u2010apoptotic gene survivin expression in malignant haematopoietic cells","volume":"21","author":"Moriai R","year":"2001","journal-title":"Anticancer Res"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-07-2130"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1053\/hupa.2002.124783"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-04-1128"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.4708"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(00)00065-5"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.20039","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.20039","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T05:51:57Z","timestamp":1700200317000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.20039"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,1]]},"references-count":20,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["10.1002\/jcla.20039"],"URL":"https:\/\/doi.org\/10.1002\/jcla.20039","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,1]]}}}