{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T18:53:41Z","timestamp":1761418421282},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,9,7]],"date-time":"2006-09-07T00:00:00Z","timestamp":1157587200000},"content-version":"vor","delay-in-days":249,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Thousands of women all over the world annually suffer of vulvovaginal candidiasis (VVC), an infection caused by yeasts, which mainly affect the mucosa of the vulva and vagina. The adherence of the yeasts to the mucosae is an essential step for colonization and predisposes the start of the infectious process. In this study, a technique capable of evaluating the adhesion of yeasts to human vaginal epithelial cells (HVEC) was employed. Twenty\u2010five vaginal yeast isolates (10 <jats:italic>Candida albicans<\/jats:italic>, nine <jats:italic>C. glabrata<\/jats:italic>, two <jats:italic>C. parapsilosis<\/jats:italic>, one <jats:italic>C. tropicalis<\/jats:italic>, two <jats:italic>Saccharomyces cerevisiae<\/jats:italic>, and one <jats:italic>Trichosporon<\/jats:italic> sp.) were evaluated. A suspension of each yeast was co\u2010incubated with HVEC obtained from a healthy donor in the ovulatory phase. After 1\u2009hr, smears were made, stained with crystal violet and Papanicolaou, and the number of yeasts that adhered to 600 HVEC was evaluated. The adhesion of <jats:italic>C. albicans<\/jats:italic> was significantly greater than that of the other species and occurred mainly in the intermediate HVEC, rather than the superficial. In addition, the proposed technique, easy to execute and of low cost showed to be reproducible and enables the determination of the adherence capacity of different isolates, whose adhesion was confirmed by scanning electron microscopy. J. Clin. Lab. Anal. 20:195\u2013203, 2006. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.20132","type":"journal-article","created":{"date-parts":[[2006,9,8]],"date-time":"2006-09-08T01:22:05Z","timestamp":1157678525000},"page":"195-203","source":"Crossref","is-referenced-by-count":14,"title":["A simplified technique for evaluating the adherence of yeasts to human vaginal epithelial cells"],"prefix":"10.1002","volume":"20","author":[{"given":"Mary Mayumi","family":"Taguti Irie","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rcia Edilaine","family":"Lopes Consolaro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terezinha","family":"Aparecida Guedes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luc\u00e9lia","family":"Donatti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliana","family":"Val\u00e9ria Patussi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terezinha Inez","family":"Estivalet Svidzinski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,9,7]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"22","article-title":"Vulvovaginitis candidi\u00e1sica","volume":"19","author":"Ziarrusta GB","year":"2001","journal-title":"Rev Iberoam Micol"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003081-199303000-00021"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2001.02152.x"},{"key":"e_1_2_1_5_2","first-page":"2002","article-title":"Correlation of Candida species and symptoms among patients with vulvovaginal candidiasis in Maring\u00e1, Paran\u00e1, Brazil","volume":"21","author":"Consolaro MEL","year":"2004","journal-title":"Rev Iberoam Micol"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1258\/095646202760159639"},{"key":"e_1_2_1_7_2","first-page":"85","volume-title":"Candida and candidiasis","author":"Odds FC","year":"1988"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.5.2939-2946.2004"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.9.5256-5258.2002"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"2662","DOI":"10.1128\/iai.61.6.2662-2669.1993","article-title":"Isolation of avirulent clones of Candida \n                  albicans with reduced ability to recognize the CR2 ligand C3d","volume":"61","author":"Franzke S","year":"1993","journal-title":"Infect Immun"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1997.tb00374.x"},{"key":"e_1_2_1_12_2","first-page":"161","volume-title":"Atlas colorido de citologia do c\u00e2ncer","author":"Takahashi M","year":"1982"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(89)90649-2"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1080\/00362178485380321"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1139\/m88-042"},{"key":"e_1_2_1_16_2","first-page":"892","volume-title":"Comprehensive cytopathology","author":"Bibbo M","year":"1997"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.2.569-572.1995"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.52.7.529"},{"key":"e_1_2_1_19_2","first-page":"891","volume-title":"The yeast. 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