{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T10:40:51Z","timestamp":1694342451259},"reference-count":6,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,9,10]],"date-time":"2007-09-10T00:00:00Z","timestamp":1189382400000},"content-version":"vor","delay-in-days":252,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We evaluated a method for determination of human cytomegalovirus (hCMV) immunoglobulin M (IgM) by CLIA, and analyzed its clinical value in patients with infectious mononucleosis. Serum samples from 407 participants were measured on an automatic CLIA analyzer. At the same time the serum samples were measured by enzyme\u2010linked immunosorbent assay (ELISA). An assessment of technological quality (methodology) in diagnostic tests demonstrated that the sensitivity of CLIA was 1.0\u2009AU\/mL and the functional sensitivity was &lt;1.6\u2009AU\/mL. The within\u2010 and between\u2010assay imprecision values for different concentrations were all under 5%. Recoveries for both methods were 96\u2013110%. The linear regression equation between expected values and measured values was y=0.644+0.986x, and correlation coefficient was 0.9991 (<jats:italic>P<\/jats:italic>&lt;0.0001). The receiver operating characteristic (ROC) curve showed that both the sensitivity and specificity of CLIA surpassed 90%. The area under the curve (AUC) was 0.990, which was significantly higher than that of ELISA (<jats:italic>P<\/jats:italic>&lt;0.05). The results indicate that CLIA is an excellent method for hCMV IgM measurement, and thus may be useful for clinical diagnoses. J. Clin. Lab. Anal. 21:255\u2013259, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.20153","type":"journal-article","created":{"date-parts":[[2007,9,10]],"date-time":"2007-09-10T21:18:15Z","timestamp":1189459095000},"page":"255-259","source":"Crossref","is-referenced-by-count":1,"title":["Evaluation of a rapid and convenient method for determination of cytomegalovirus immunoglobulin M"],"prefix":"10.1002","volume":"21","author":[{"given":"Jiajun","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junchuan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youshan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyu","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Hang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,9,10]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-9942-2"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.1.86"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/142.5.793"},{"key":"e_1_2_1_5_2","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1128\/jcm.21.6.930-935.1985","article-title":"Immunoglobulin M antibodies detected by enzyme\u2010linked immunosorbent assay and radioimmunoassay in the diagnosis of cytomegalovirus infection in pregnant women and newborn infants","volume":"21","author":"Stagno S","year":"1985","journal-title":"J Clin Microbiol"},{"key":"e_1_2_1_6_2","first-page":"297","article-title":"Cytomegalovirus IgM antibody detection: comparison of five assays","volume":"16","author":"Kraat YJ","year":"1993","journal-title":"New Microbiol"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.30.8.1937-1941.1992"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.20153","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.20153","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.20153","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.20153","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.20153","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T03:39:36Z","timestamp":1694230776000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.20153"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1]]},"references-count":6,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,1]]}},"alternative-id":["10.1002\/jcla.20153"],"URL":"https:\/\/doi.org\/10.1002\/jcla.20153","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,1]]}}}