{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T06:26:13Z","timestamp":1778048773548,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2007,7,9]],"date-time":"2007-07-09T00:00:00Z","timestamp":1183939200000},"content-version":"vor","delay-in-days":189,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A polymerase chain reaction (PCR)\u2010based enzyme\u2010linked immunosorbent assay (ELISA) methodology was developed to characterize <jats:italic>Neisseria gonorrhoeae porB<\/jats:italic> gene variable regions (VR); the methodology was evaluated in comparison to <jats:italic>porB<\/jats:italic> VR typing by checkerboard hybridization. Clinical noncultured samples from 35 men who have sex with men (MSM), positive by nucleic amplification assays for <jats:italic>N. gonorrhoeae<\/jats:italic>, were typed using a panel of 40 oligonucleotide probes to <jats:italic>porB<\/jats:italic> VRs and compared to checkerboard hybridization. Complete concordance was observed between the two methods at PIB VRs 1, 3, and 7. At the more degenerate VRs 5 and 6, PCR ELISA resulted in obtaining more typeable VRs than checkerboard hybridization due to single nucleotide mismatches. By PCR ELISA, two predominant PIB <jats:italic>porB<\/jats:italic> types were identified in 58% of the samples and the remaining 16 samples had one of six other <jats:italic>porB<\/jats:italic> types. Both PCR ELISA and checkerboard hybridization methods of <jats:italic>porB<\/jats:italic> VR typing allowed characterization of <jats:italic>N. gonorrhoeae<\/jats:italic> from noncultured clinical samples including throat and rectal swabs and discriminated <jats:italic>N. gonorrhoeae<\/jats:italic> from <jats:italic>N. meningitidis<\/jats:italic> present in some of the samples. PCR ELISA is a rapid, relatively inexpensive and alternative molecular typing method for <jats:italic>N. gonorrhoeae<\/jats:italic>, suitable for use in conjunction with molecular diagnostic tests. J. Clin. Lab. Anal. 21:237\u2013243, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.20173","type":"journal-article","created":{"date-parts":[[2007,7,9]],"date-time":"2007-07-09T23:51:44Z","timestamp":1184025104000},"page":"237-243","source":"Crossref","is-referenced-by-count":8,"title":["Evaluation of <i>PorB<\/i> variable region typing of <i>Neisseria gonorrhoeae<\/i> using PCR\u2010ELISA in samples collected from men who have sex with 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