{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:24:05Z","timestamp":1788870245395,"version":"build-2803163510"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2007,9,10]],"date-time":"2007-09-10T00:00:00Z","timestamp":1189382400000},"content-version":"vor","delay-in-days":252,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Greiner Bio-One Brazil"},{"DOI":"10.13039\/100001316","name":"Abbott Laboratories","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100001316","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Rubella infection is an exanthematic disease, with high prevalence in the adult population. The only modality of disease that causes serious consequences is congenital rubella syndrome (CRS), which happens when a pregnant woman seronegative to rubella virus acquires the infection during early pregnancy. Due to the lack of signals and characteristic symptoms of disease, diagnosis of rubella is based essentially on laboratory tests: antibodies detection and\/or virus isolation. Results of serologic tests should always be interpreted with caution, because they can be affected by the quality of blood samples, processing and storage of sera, the equipment and reagents used to perform tests, and finally by the technical expertise and training of biologists. The collection tubes with gel seem to facilitate serum separation, but on the other hand gels can retain and consequently decrease antibody titers. Therefore, we decided to investigate whether the use of collection tubes containing gel separator might interfere with rubella virus antibody detection in blood samples from children. We did not observe statistically significant differences with respect to rubella virus antibody detection (immunoglobulin M [IgM] and immunoglobulin G [IgG]) for samples collected in tubes with or without gel separator, from the two evaluated manufacturers. J. Clin. Lab. Anal. 21:330\u2013334, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.20194","type":"journal-article","created":{"date-parts":[[2007,9,10]],"date-time":"2007-09-10T17:18:08Z","timestamp":1189444688000},"page":"330-334","source":"Crossref","is-referenced-by-count":4,"title":["Collection tubes with or without gel separator did not interfere with detection of rubella virus antibodies IgM and IgG"],"prefix":"10.1002","volume":"21","author":[{"given":"L.C.","family":"Oliveira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.H.","family":"Kawasato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.S.","family":"Otta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.P.","family":"Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.S.","family":"Okay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,9,10]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4899-0036-4_27"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(82)80761-0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.10.5117-5121.2005"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(85)80680-6"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.32.2.128"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00474-1"},{"key":"e_1_2_1_8_2","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1128\/CDLI.8.4.785-787.2001","article-title":"Antenatal screening goes hepatitis B and antibodies to toxoplasma gondii and rubella virus: evaluation of two commercial immunoassay systems","volume":"8","author":"Diepersloot RJA","year":"2001","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.10.4818-4820.2004"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.325.7356.147"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890360308"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(80)92419-8"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1246-7820(00)00009-4"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2825(1997)11:4<232::AID-JCLA10>3.0.CO;2-X"},{"key":"e_1_2_1_15_2","volume-title":"Evaluation and performance criteria for multiple component test product intended for detection and quantification of rubella IgG antibody. Tentative guideline. NCCLS Document I\/LA6\u2010T","author":"National Committee for Clinical Laboratory Standards","year":"1992"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9071(200001)60:1<43::AID-JMV8>3.0.CO;2-W"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1093\/clinchem\/45.2.313","article-title":"False\u2010positive hCG assays results leading to unnecessary surgery and chemotherapy and needless occurrences of diabetes and coma","volume":"45","author":"Cole LA","year":"1999","journal-title":"Clin Chem"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1258\/000456303322326498"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.57.1.104"},{"key":"e_1_2_1_20_2","unstructured":"Medical Device Alert. Medicines and Healthcare Products Regulatory Agency. Ref. MDA\/2004\/0049. 28 October 2004. Medicine and Healthcare Products Regulatory Agency (MHRA) United Kingdom."},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.043349"},{"key":"e_1_2_1_22_2","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1093\/clinchem\/45.1.62","article-title":"Evaluation of interferences in rate and fixed\u2010time nephelometric assays of specific serum proteins","volume":"45","author":"Bossuyt X","year":"1999","journal-title":"Clin Chem"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(85)90119-3"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.20194","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.20194","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.20194","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.20194","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T23:39:27Z","timestamp":1694216367000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.20194"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1]]},"references-count":22,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,1]]}},"alternative-id":["10.1002\/jcla.20194"],"URL":"https:\/\/doi.org\/10.1002\/jcla.20194","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,1]]}}}