{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T21:58:54Z","timestamp":1762898334598},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2007,11,16]],"date-time":"2007-11-16T00:00:00Z","timestamp":1195171200000},"content-version":"vor","delay-in-days":319,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We evaluated the diagnostic value of Flinders Technology Associates (FTA) filter paper together with polymerase chain reaction (PCR) for detection of <jats:italic>Pneumocystis jirovecii<\/jats:italic> (<jats:italic>carinii<\/jats:italic>) from induced sputum (IS) and bronchoalveolar lavage fluid (BALF) samples. The study involved 162 patients with clinical diagnosis of pneumocystis pneumonia (PcP) of human immunodeficiency virus\/acquired immune deficiency syndrome (HIV\/AIDS) patients and other immunocompromised patients. <jats:italic>P. jirovecii<\/jats:italic> cysts or trophozoites were detected in IS and BALF by cytological method. The mitochondrial 5S ribosomal ribonucleic acid (rRNA) gene of <jats:italic>P. jirovecii<\/jats:italic> was amplified from these samples by using FTA filters together with a one\u2010step PCR method (FTA\u2010PCR). With the FTA\u2010PCR method, the sensitivity and specificity of the test compared to microscopic examination were 67% and 90% for IS, while they were 67% and 91% for BALF, respectively. The sensitivity and specificity of the FTA\u2010PCR test was also comparable to PCR with the conventional deoxyribonucleic acid (DNA) extraction method. We concluded that FTA\u2010PCR is useful to detect <jats:italic>P. jirovecii<\/jats:italic> in noninvasive IS. J. Clin. Lab. Anal. 21:382\u2013386, 2007. \u00a9 2007 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.20200","type":"journal-article","created":{"date-parts":[[2007,11,17]],"date-time":"2007-11-17T01:46:38Z","timestamp":1195263998000},"page":"382-386","source":"Crossref","is-referenced-by-count":13,"title":["Flinders technology associates (FTA) filter paper\u2013based DNA extraction with polymerase chain reaction (PCR) for detection of <i>Pneumocystis jirovecii<\/i> from respiratory specimens of immunocompromised 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