{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T21:33:18Z","timestamp":1767994398923,"version":"3.49.0"},"reference-count":10,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2008,1,16]],"date-time":"2008-01-16T00:00:00Z","timestamp":1200441600000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2008,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Wegener's granulomatosis (WG) is one type of systemic small vessel vasculitis and antineutrophil cytoplasmic antibodies (ANCA) have become an established diagnostic tool for systemic vasculitis. The sensitivity and specificity of anti\u2010PR3 (proteinase 3) capture enzyme\u2010linked immunosorbent assay (ELISA) in diagnosing WG were investigated, as well as the correlation with the indirect immunofluorescence test (IIFT). Sera from 72 patients with WG, 100 disease controls, and 206 healthy blood donors were investigated for anti\u2010PR3 and cytoplasmic ANCA (cANCA) by anti\u2010PR3 classic ELISA, anti\u2010PR3 capture ELISA, and IIFT. The sensitivity of anti\u2010PR3 classic ELISA and capture ELISA in diagnosing WG was 74% and 87.5% separately. The specificity of the two ELISA was identical (100%). For the combination of IIFT with anti\u2010PR3 capture ELISA, the sensitivity for WG patients was up to 91.6%. The sensitivity of anti\u2010PR3 capture ELISA is superior to anti\u2010PR3 classic ELISA, and the correlation between anti\u2010PR3 capture ELISA and IIFT is also more superior. For suspected WG, anti\u2010PR3 capture ELISA and IIFT should be applied in parallel. J. Clin. Lab. Anal. 22:73\u201376, 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.20204","type":"journal-article","created":{"date-parts":[[2008,1,17]],"date-time":"2008-01-17T03:17:59Z","timestamp":1200539879000},"page":"73-76","source":"Crossref","is-referenced-by-count":9,"title":["Clinical relevance of anti\u2010PR3 capture ELISA in diagnosing Wegener's granulomatosis"],"prefix":"10.1002","volume":"22","author":[{"given":"Zhenru","family":"Feng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiyan","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baohuan","family":"Sui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,1,16]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(85)91147-X"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/346520a0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780330807"},{"key":"e_1_2_1_5_2","first-page":"15","article-title":"Laboratory diagnostics of antineutrophil cytoplasmic antibodies (ANCA)","volume":"38","author":"Li YZ","year":"2003","journal-title":"Chin Med J"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/111.4.507"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.149.4.1409"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(94)00330-Y"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(93)90223-T"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(92)90066-3"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V81.8.1996.1996"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.20204","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.20204","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T07:26:49Z","timestamp":1700033209000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.20204"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,1]]},"references-count":10,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2008,1]]}},"alternative-id":["10.1002\/jcla.20204"],"URL":"https:\/\/doi.org\/10.1002\/jcla.20204","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,1]]}}}