{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:50:42Z","timestamp":1791179442632,"version":"4.1.0"},"reference-count":13,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,1,12]],"date-time":"2009-01-12T00:00:00Z","timestamp":1231718400000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Background<\/jats:italic>: Aim of this study was to establish the method yielding the highest sensitivity routinely used to determine fetal RhD type and gender from maternal cell\u2010free plasma DNA in different periods of gestation.<\/jats:p><jats:p><jats:italic>Methods<\/jats:italic>: Plasma DNA concentrations were measured from 46 pregnant women in different gestational periods and tested for RhD using three different PCR methods on exon 7: Thermal Cycler, Taqman method on LightCycler, and melting curve analysis on LightCycler. In addition, fetal gender was determined by PCR. Cell\u2010free plasma DNA was measured in 100 healthy volunteers as a reference group.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>: The mean value of cell\u2010free plasma DNA in the reference group was 10.9\u2009pg\/\u00b5L mean, (standard deviation (SD): 3.66) in 50 healthy women and 12.7\u2009pg\/\u00b5L (SD: 8.2) in 50 healthy men. In the firsttrimester of pregnancy cell\u2010free plasmaDNA was 14.9\u2009pg\/\u00b5L mean, (SD: 4.2), in the second trimester 15.4\u2009pg\/\u00b5L mean, (SD: 4.96), and the maximum was achieved in the third trimester of pregnancy 15.6\u2009pg\/\u00b5l mean, (SD: 6.49). TaqMan probes had the same accuracy, when compared with Thermal Cycler technology (46 samples, 6 failures). Using real\u2010time PCR with melting curve analysis 12 of 17 samples were correctly tested. Gender determination was correctly in 41 of 46 samples.<\/jats:p><jats:p><jats:italic>Conclusion<\/jats:italic>: RhD determinations with TaqMan and Thermal Cycler technology are useful methods for fetal RhD prediction. To increase the accuracy of RhD determination it is necessary to test on other exons in addition. J. Clin. Lab. Anal. 23:24\u201328, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.20282","type":"journal-article","created":{"date-parts":[[2009,1,12]],"date-time":"2009-01-12T22:00:05Z","timestamp":1231797605000},"page":"24-28","source":"Crossref","is-referenced-by-count":8,"title":["Comparison of PCR methods for detecting fetal RhDin maternal plasma"],"prefix":"10.1002","volume":"23","author":[{"given":"Johanna","family":"Atamaniuk","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karl M.","family":"Stuhlmeier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alireza","family":"Karimi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias M.","family":"Mueller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1999.39299154731.x"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.2000.tb11674.x"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s102380200010"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)02174-0"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1318.019"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2001.tb03872.x"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1071-5576(98)00051-3"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0004-8666.2003.00011.x"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2006.07.033"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2006.01044.x"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1248"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01175.x"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1159\/000085085"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.20282","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.20282","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,14]],"date-time":"2023-11-14T21:04:43Z","timestamp":1699995883000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.20282"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1]]},"references-count":13,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2009,1]]}},"alternative-id":["10.1002\/jcla.20282"],"URL":"https:\/\/doi.org\/10.1002\/jcla.20282","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,1]]}}}