{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T00:07:08Z","timestamp":1700006828438},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2009,1,12]],"date-time":"2009-01-12T00:00:00Z","timestamp":1231718400000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The human immune response to tuberculosis (TB) is especially mediated by T CD4<jats:sup>+<\/jats:sup>lymphocytes. However, more studies are needed in order to understand the exact role of each cytokine in the mechanisms for cures. In this article, our aim was to analyze the production of TNF\u2010\u03b1, IL\u201010, and IFN\u2010\u03b3 in peripheral blood mononuclear cells (PBMCs) among the household contacts of common primary TB cases, with or without histories of active TB infection, who were negative to parasitological and HIV tests. In order to characterize the cytokine production, PBMCs from these groups werestimulated with whole\u2010protein extract of <jats:italic>M. tuberculosis<\/jats:italic> (WPE) antigen (rAgTb) for 24 and 48\u2009hr. The culture supernatants were collected and IFN\u2010\u03b3, TNF\u2010\u03b1, and IL\u201010 were assayed using capture ELISA. There were no statistical differences between primary TB cases and their household contacts with or without previous histories of lung TB. Our results suggest that T memory cells, T regulatory cells, and the Th1\/Th2 dichotomy may be responsible for the results described in this article. Further studies are currently underway. J. Clin. Lab. Anal. 23:57\u201362, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.20290","type":"journal-article","created":{"date-parts":[[2009,1,12]],"date-time":"2009-01-12T22:00:05Z","timestamp":1231797605000},"page":"57-62","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of memory immune response to mycobacterium extract among household contact of tuberculosis cases"],"prefix":"10.1002","volume":"23","author":[{"given":"Y.V.N.","family":"Cavalcanti","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.R.A.","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.C.","family":"Reis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.L.G.","family":"Ramos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.F.","family":"Luna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.J.M.","family":"Nascimento","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Lucena\u2010Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,1,12]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.293.22.2767"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/9781555818357.ch24"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.19.1.93"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/315114"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.02.002"},{"key":"e_1_2_1_7_2","first-page":"213","article-title":"Immunology of tuberculosis","volume":"120","author":"Raja A","year":"2004","journal-title":"Indian J Med Res"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.3.1847-1855.2001"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.6.2249"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"3482","DOI":"10.1128\/iai.61.8.3482-3489.1993","article-title":"Cytokine production at the site of disease in human tuberculosis","volume":"61","author":"Barnes Pf","year":"1994","journal-title":"Infect Immun"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.10.5473-5476.1999"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2003.08.010"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(00)00199-5"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(00)01919-3"},{"key":"e_1_2_1_15_2","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1128\/IAI.66.1.176-180.1998","article-title":"Cytokine Profiles for peripheral blood lymphocytes from patients with active pulmonary tuberculosis and healthy household contacts in response to the 30\u2010kilodalton antigen of Mycobacterium tuberculosis","volume":"66","author":"Torres M","year":"1998","journal-title":"Infect Immun"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1997.tb01046.x"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1988.tb02338.x"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0037-86822008000100003"},{"key":"e_1_2_1_19_2","first-page":"283","article-title":"The sedimentation\u2013concetration method in Schistosomiasis mansoni","volume":"9","author":"Hoffman WA","year":"1934","journal-title":"The Puerto Rico Journal of Public Health"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3024.2002.00600.x"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117611"},{"key":"e_1_2_1_22_2","first-page":"299","article-title":"Comparative and prospective study of different immune parameters in healthy subjects at risk for tuberculosis and in tuberculosis patients","volume":"9","author":"Portales\u2010Perez DP","year":"2002","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1998.00561.x"},{"key":"e_1_2_1_24_2","doi-asserted-by":"crossref","first-page":"5673","DOI":"10.1128\/iai.62.12.5673-5678.1994","article-title":"Immune responsiveness and lymphokine production in patients with tuberculosis and healthy controls","volume":"62","author":"S\u00e1nchez FO","year":"1994","journal-title":"Infect Immun"},{"key":"e_1_2_1_25_2","doi-asserted-by":"crossref","first-page":"4641","DOI":"10.4049\/jimmunol.150.10.4641","article-title":"Analysis of cytokine production by Mycobacterium\u2010reactive T cells. 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