{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T05:03:50Z","timestamp":1773378230426,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,3,13]],"date-time":"2009-03-13T00:00:00Z","timestamp":1236902400000},"content-version":"vor","delay-in-days":71,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The fungus <jats:italic>Paracoccidioides brasiliensis<\/jats:italic> is the pathogen of paracoccidioidomycosis (PCM), a systemic mycosis prevalent in Latin America. The loop\u2010mediated isothermal amplification method (LAMP) was used in this study to detect the presence of <jats:italic>P. brasiliensis<\/jats:italic> in sputa samples from patients with chronic PCM, suspected PCM, and a negative control. The target <jats:italic>P. brasiliensis<\/jats:italic> gp43 gene was amplified in less than 4\u2009hr in 11 of 18 sputa samples tested. The LAMPmethod had the advantage of speed and simplicity compared with the classic diagnostic methods such as the histopathological test or biological material culture and did not require sophisticated technical apparatus. It would be an important aid in cases where immediate treatment would mean patient survival, especially in immune\u2010suppressed patients. J. Clin. Lab. Anal. 23:139\u2013143 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.20304","type":"journal-article","created":{"date-parts":[[2009,3,13]],"date-time":"2009-03-13T13:15:58Z","timestamp":1236950158000},"page":"139-143","source":"Crossref","is-referenced-by-count":28,"title":["Detection of <i>Paracoccidioides brasiliensis<\/i> gp43 Gene in Sputa by Loop\u2010Mediated Isothermal Amplification Method"],"prefix":"10.1002","volume":"23","author":[{"given":"B. 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