{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:19:31Z","timestamp":1791253171982,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,7,21]],"date-time":"2009-07-21T00:00:00Z","timestamp":1248134400000},"content-version":"vor","delay-in-days":201,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Introduction<\/jats:italic>: Different methods are available for diagnosis of visceral leishmaniasis (VL), among them the urine\u2010based antigen detection assay, latex agglutination test (Katex), is a recently developed one. The main drawback of the test is false\u2010positive reactivity in some of healthy individuals. The false positivity of the test can be removed by boiling the urine sample for 5\u2009min before testing. In this study an attempt was made to improve Katex by removing unpleasant boiling process, which also decreases field applicability of the test.<\/jats:p><jats:p><jats:italic>Methods<\/jats:italic>: False\u2010positive and true\u2010positive urine samples were collected from VL patients and healthy individuals. Both samples were then treated by reagents including, sodium dodecyl sulfate, trichloroacetic acid, dithiothreitol (DTT), sulphosalicylic acid and also heating at 56\u00b0C.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>: Findings of this study showed that DTT pretreatment significantly reduced the rate of false\u2010positive reactivity of Katex where 73% of false\u2010positive urine samples changed to negative after DTT treatment. However, the DTT treatment reduced the rate of true positivity by 14%.<\/jats:p><jats:p><jats:italic>Conclusion<\/jats:italic>: These data indicate that DTT can be used to eliminate nonspecific reactivity in the Katex. This will improve the performance of Katex and make it a more convenient and field applicable test. J. Clin. Lab. Anal. 23:202\u2013205, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.20312","type":"journal-article","created":{"date-parts":[[2009,7,21]],"date-time":"2009-07-21T21:20:54Z","timestamp":1248211254000},"page":"202-205","source":"Crossref","is-referenced-by-count":14,"title":["Improvement of the newly developed latex agglutination test (Katex) for diagnosis of visceral lieshmaniasis"],"prefix":"10.1002","volume":"23","author":[{"given":"Gholam Reza","family":"Hatam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Amin","family":"Ghatee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Mohammad Hossein","family":"Hossini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bahador","family":"Sarkari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,7,21]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2005.07.001"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)10178-2"},{"key":"e_1_2_1_4_2","unstructured":"World Health Organization. The World Health Report. World Health Organization Geneva Switzerland 2001."},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.4103\/0022-3859.927"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2005.01533.x"},{"key":"e_1_2_1_7_2","first-page":"951","article-title":"Laboratory diagnosis of visceral leishmaniasis","volume":"9","author":"Sundar S","year":"2002","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_2_1_8_2","first-page":"899","article-title":"Evaluation of a latex agglutination test (KAtex) for detection of Leishmania antigen in urine of patients with HIV\u2010Leishmania coinfection: Value in diagnosis and post\u2010treatment follow\u2010up","volume":"23","author":"Riera C","year":"2004","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(02)00043-8"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(00)00155-8"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"844","DOI":"10.26719\/2003.9.4.844","article-title":"Field evaluation of latex agglutination test for detecting urinary antigens in visceral leishmaniasis in Sudan","volume":"9","author":"El\u2010Safi SH","year":"2003","journal-title":"East Mediterr Health J"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2007.09.003"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2004.01251.x"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2007.05.002"},{"key":"e_1_2_1_15_2","first-page":"101","article-title":"Evaluation of latex agglutination test (Katex) for the detection of urinary antigens in human visceral leishmaniasis","volume":"4","author":"Malaei S","year":"2006","journal-title":"J Tehran Un Med Sci"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.1989.tb02194.x"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1093\/clinchem\/46.3.392","article-title":"Total protein determination in urine: Elimination of a differential response between the Coomassie blue and pyrogallol red protein dye\u2010binding assays","volume":"46","author":"Marshall T","year":"2000","journal-title":"Clin Chem"},{"key":"e_1_2_1_18_2","first-page":"396","article-title":"Conversion to positivity of false negative latex\/hepatitis associated antigen agglutination test upon treatment of sera with EDTA or trypsin","volume":"144","author":"Spano C","year":"1972","journal-title":"Z Immunitatsforsch Exp Klin Immunol"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/34.4.767a"},{"key":"e_1_2_1_20_2","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1128\/jcm.5.1.31-33.1977","article-title":"Rheumatoid factor: A cause of false positive histoplasmin latex agglutination","volume":"5","author":"Oxenhandler RW","year":"1977","journal-title":"J Clin Microbiol"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.20312","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.20312","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T07:36:04Z","timestamp":1695972964000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.20312"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1]]},"references-count":19,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2009,1]]}},"alternative-id":["10.1002\/jcla.20312"],"URL":"https:\/\/doi.org\/10.1002\/jcla.20312","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,1]]}}}