{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T10:41:34Z","timestamp":1756809694353},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,9,22]],"date-time":"2009-09-22T00:00:00Z","timestamp":1253577600000},"content-version":"vor","delay-in-days":264,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In this study, we evaluated a new immunoassay for the automated detection of anti\u2010<jats:italic>toxoplasma<\/jats:italic> IgG and IgM with Immulite 2000<jats:sup>\u00ae<\/jats:sup> (DPC\u2010Siemens, La Garenne\u2010Colombes, France). We tested 280 sera from 112 patients with past infection (PI), 40 PI with residual IgM, 75 seronegatives, 16 infants (31 sera) monitored for neonatal screening of congenital toxoplasmosis, and 13 patients with recent seroconversion (SC) (22 sequential sera). Detection sensitivity and specificity for IgG were 99 and 100%, respectively. IgG titers obtained with Immulite 2000<jats:sup>\u00ae<\/jats:sup> were higher than with Vidas<jats:sup>\u00ae<\/jats:sup> (BioM\u00e9rieux, Marcy I'Etoile, France) and Access<jats:sup>\u00ae<\/jats:sup> (Beckman\u2010Coulter, Villepinte, France) (paired Wilcoxon test <jats:italic>z<\/jats:italic>=4.44 and <jats:italic>z<\/jats:italic>=3.67, respectively, <jats:italic>P<\/jats:italic>&lt;0.001). IgM specificity was 100%. Detection sensitivity for IgM was 100% in the SC group, 86% in congenitally infected infants, and 75% in PI with persistent IgM. IgM detection seemed less prolonged in time than with the IgM Access<jats:sup>\u00ae<\/jats:sup> and ISAGA<jats:sup>\u00ae<\/jats:sup> IgM techniques. J. Clin. Lab. Anal. 23:336\u2013340, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.20338","type":"journal-article","created":{"date-parts":[[2009,9,22]],"date-time":"2009-09-22T21:37:14Z","timestamp":1253655434000},"page":"336-340","source":"Crossref","is-referenced-by-count":10,"title":["Evaluation of DPC immulite 2000<sup>\u00ae<\/sup><i>Toxoplasma<\/i> quantitative IgG\/IgM kits for automated toxoplasmosis serology with immulite 2000<sup>\u00ae<\/sup>"],"prefix":"10.1002","volume":"23","author":[{"given":"Florence","family":"Robert\u2010Gangneux","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chantal","family":"Bourhis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sylviane","family":"Chevrier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Pierre","family":"Gangneux","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,9,22]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199406303302604"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.7.2504-2508.2002"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2005.04.005"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9378(99)70224-3"},{"key":"e_1_2_1_6_2","first-page":"165","article-title":"Congenital toxoplasmosis: Specific IgM in fetal blood, cord blood and in the newborn","volume":"54","author":"Fricker\u2010Hidalgo H","year":"1996","journal-title":"Ann Biol Clin"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0223(199711)17:11<1047::AID-PD192>3.0.CO;2-Q"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199409153311102"},{"key":"e_1_2_1_9_2","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1128\/jcm.34.3.579-583.1996","article-title":"Early neonatal diagnosis of congenital toxoplasmosis: Value of comparative enzyme\u2010linked immunofiltration assay immunological profiles and anti\u2010Toxoplasma gondii immunoglobulin M or IgA immunocapture and implications for postnatal therapeutic strategies","volume":"34","author":"Pinon JM","year":"1996","journal-title":"J Clin Microbiol"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"2893","DOI":"10.1128\/JCM.37.9.2893-2898.1999","article-title":"Congenital toxoplasmosis: Value of prenatal diagnosis and early postnatal diagnosis. 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