{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T16:10:12Z","timestamp":1700583012208},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,1,19]],"date-time":"2010-01-19T00:00:00Z","timestamp":1263859200000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The development of antimicrobial resistance of <jats:italic>Neisseria gonorrhoeae<\/jats:italic> arising from wide dissemination of resistant clones is a major global health problem. In this study, a total of 235 isolates of <jats:italic>N. gonorrhoeae<\/jats:italic> isolated from patients of Bangrak Hospital were tested for their antibiotic susceptibilities to penicillin, norfloxacin, ofloxacin, ciprofloxacin, spectinomycin, and ceftriaxone. Mutation (Ser\u201091) in the quinolone resistance determining regions of <jats:italic>gyrA<\/jats:italic> and random amplification of the polymorphic DNA polymerase chain reaction (RAPD\u2010PCR) were examined from 145 isolates. Among these, 55 isolates were obtained during January\u2013March 2000, 46 isolates during January\u2013March 2002, and 44 isolates during October\u2013December 2002. The occurrence of combination resistance between penicillin and quinolone was 20% in January\u2013March 2000, which was increased to 57.8% during the period of October\u2013December 2002 (<jats:italic>P<\/jats:italic>&lt;0.0001). Mutation of Ser\u201091 in <jats:italic>gyrA<\/jats:italic> could be directly linked with the resistance or declining of susceptibility to ciprofloxacin. Using RAPD\u2010PCR, we could classify the 145 isolates into 4 and 5 groups by primers D11344 (5\u2032\u2010AGTGAATTCGCGGTGAGATGCCA\u20103\u2032) and D8635 (5\u2032\u2010GAGCGGCCAAAGGGAGCA GAC\u20103\u2032), respectively. Combination of the data obtained from these two primers produced 11 fingerprint groups. Our findings conclude that monitoring of the Ser\u201091 mutation of <jats:italic>gyrA<\/jats:italic> and RAPD\u2010PCR methods are most useful for epidemiological screening. J. Clin. Lab. Anal. 24:31\u201337, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.20355","type":"journal-article","created":{"date-parts":[[2010,1,19]],"date-time":"2010-01-19T19:56:57Z","timestamp":1263931017000},"page":"31-37","source":"Crossref","is-referenced-by-count":5,"title":["Antibiograms and randomly amplified polymorphic DNA\u2010polymerase chain reactions (RAPD\u2010PCR) as epidemiological markers of gonorrhea"],"prefix":"10.1002","volume":"24","author":[{"given":"Ratana","family":"Lawung","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angkana","family":"Charoenwatanachokchai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rungrot","family":"Cherdtrakulkiat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sivarak","family":"Thammapiwan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tharinda","family":"Mungniponpan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leif","family":"B\u00fclow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virapong","family":"Prachayasittikul","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,1,19]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"S12","article-title":"Global prevalence and incidence estimates of selected curable STDs","volume":"74","author":"Gerbase AC","year":"1998","journal-title":"Sex Transm Infect"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)90001-0"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/430787"},{"key":"e_1_2_1_5_2","unstructured":"Fact sheets on young people and HIV\/AIDS. 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