{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T20:34:55Z","timestamp":1777581295655,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,3,23]],"date-time":"2010-03-23T00:00:00Z","timestamp":1269302400000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have developed a new method based on specific primer extension reactions coupled with plate hybridization for high\u2010throughput genotyping of single\u2010base mutations. To improve the switching characteristics of the primer extension reaction, we introduced an artificial mismatch two bases upstream of the 3\u2032\u2010terminal base in the detection primers. A set of primers that correspond to wild\u2010type and mutant DNA segments can be used to accurately analyze single\u2010base mutations. The termini of these primers are at the mutation positions. The primer extension products produced by polymerase chain reaction (PCR) were captured by an oligonucleotide probe immobilized on the surface of microtiter wells and were detected by a colorimetric assay using the streptavidin\u2010conjugated horseradish peroxidase. We used the new method to genotype 96 individuals for 211G&gt;A (G71R) and 119 for 1456T&gt;G (Y486D) in the UDP\u2010glucuronosyltransferase1A1 gene; the results were completely concordant with those found by direct sequencing. The proposed method includes ordinary PCR and a microplate assay format, and may be used in routine laboratory tests. J. Clin. Lab. Anal. 24:85\u201391, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.20359","type":"journal-article","created":{"date-parts":[[2010,3,23]],"date-time":"2010-03-23T20:19:59Z","timestamp":1269375599000},"page":"85-91","source":"Crossref","is-referenced-by-count":7,"title":["High\u2010throughput single\u2010base mismatch detection for genotyping of UDP\u2010glucuronosyltransferase (UGT1A1) with probe capture assay coupled with modified allele\u2010specific primer extension reaction (MASPER)"],"prefix":"10.1002","volume":"24","author":[{"given":"Osamu","family":"Kisaki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seiji","family":"Kato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kohei","family":"Shinohara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisahide","family":"Hiura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomohiro","family":"Samori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Sato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,3,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0997-21"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-1004(1998)12:4<221::AID-HUMU1>3.0.CO;2-I"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0004.1999.560401.x"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/75360"},{"key":"e_1_2_1_6_2","doi-asserted-by":"crossref","first-page":"710","DOI":"10.2144\/00284st07","article-title":"Electrophoretic detection of single\u2010nucleotide polymorphisms","volume":"28","author":"See D","year":"2000","journal-title":"Biotechniques"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0198-49"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1050-3862(98)00018-7"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.7.2503"},{"key":"e_1_2_1_10_2","first-page":"105","article-title":"Rapid nonradioactive detection of mutations in the human genome by allele\u2010specific amplification","volume":"114","author":"Okayama H","year":"1989","journal-title":"J Lab Clin Med"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/354204a0"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/7044"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/5270"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1996.9913"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2000.4493"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.10.6.853"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.1093\/clinchem\/43.7.1114","article-title":"Methods for detection of point mutations: Performance and quality assessment. 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