{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:08:45Z","timestamp":1761743325002},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,3,23]],"date-time":"2010-03-23T00:00:00Z","timestamp":1269302400000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We describe the history and current implementation of an inexpensive thin layer chromatography (TLC) method, vertical sandwich\u2010type continuous\/evaporative TLC with fixed mobile phase volume, that is convenient for detecting and identifying reducing sugars of clinical relevance in the paper\u2010borne blood and urine samples collected in neonatal screening programmes. This method facilitates screening by providing a considerable degree of standardization of chromatographic results. Among some 555,000 newborns to which it has been applied, it has detected 10 cases of classical galactosaemia, 7 cases of galactokinase deficiency, 2 cases of glucosuria, and 3 cases of transitory neonatal diabetes mellitus; the only false negatives we are aware of were two cases of galacto\u20104\u2010epimerase deficiency detected by tandem mass spectrometry. Screening for sugars in urine has allowed the detection of galactosaemia when the accompanying blood sample was invalid because of transfusion or parenteral feeding. The conclusion is that this inexpensive procedure is very useful for the detection of relevant metabolopathies in circumstances where others fail. J. Clin. Lab. Anal. 24:106\u2013112, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.20371","type":"journal-article","created":{"date-parts":[[2010,3,23]],"date-time":"2010-03-23T20:20:13Z","timestamp":1269375613000},"page":"106-112","source":"Crossref","is-referenced-by-count":7,"title":["Vertical sandwich\u2010type continuous\/evaporative TLC with fixed mobile phase volume for separating sugars of clinical relevance in paper\u2010borne urine and blood samples in newborn screening"],"prefix":"10.1002","volume":"24","author":[{"given":"J.R.","family":"Alonso\u2010Fernandez","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.I.","family":"Carpinteiro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Baleato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Fidalgo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,3,23]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1958.72990350004005a"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/5.6.603"},{"key":"e_1_2_1_4_2","first-page":"707","article-title":"Action of amines on quinolinic acid","volume":"11","author":"Das AK","year":"1934","journal-title":"J Ind Chem Soc"},{"key":"e_1_2_1_5_2","first-page":"50","volume-title":"Phenylketonuria and Allied Metabolic Diseases","author":"Woolf LI.","year":"1967"},{"key":"e_1_2_1_6_2","first-page":"233","volume-title":"Advances in Neonatal Screening","author":"Alonso\u2010Fern\u00e1ndez JR","year":"1987"},{"key":"e_1_2_1_7_2","unstructured":"Gim\u00e9nez\u2010SerratM Julve P\u00e9rezE.Protocolo de dos m\u00e9todos de detecci\u00f3n cualitativa de sustancias reductoras en orina impregnada en papel. Dispensario M\u00e9dico Diagn\u00f3stico y Prevenci\u00f3n Caja de Ahorros y Monte de Piedad de Zaragoza Arag\u00f3n y la Rioja. Personal communication June 15th1993."},{"key":"e_1_2_1_8_2","first-page":"11","volume-title":"Review of Physiologic Chemistry","author":"Harper HA","year":"1969"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-9120(78)80016-2"},{"key":"e_1_2_1_10_2","first-page":"243","volume-title":"Advances in Neonatal Screening","author":"Ng WG","year":"1987"},{"key":"e_1_2_1_11_2","first-page":"77","volume-title":"Laboratory Methods for Neonatal Screening","author":"Webster D","year":"1993"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2006-1783"},{"key":"e_1_2_1_13_2","first-page":"133","article-title":"Tr\u00eda Neonatal de Galactosemia: Situaci\u00f3n del Ensayo en Orina","volume":"1","author":"Alonso\u2010Fern\u00e1ndez JR","year":"2008","journal-title":"Rev Lab Clin"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)88207-2"},{"key":"e_1_2_1_15_2","first-page":"129","volume-title":"Fundamentals of Thin\u2010layer Chromatography (Planar Chromatography)","author":"Geiss F.","year":"1987"},{"key":"e_1_2_1_16_2","unstructured":"Alonso\u2010Fern\u00e1ndezJR QuintasR Pe\u00f1aJ FragaJM.A method for the screening separation identification and semiquantitative assessment of sugars of metabolic interest in urine samples impregnated on paper. International Symposium on Inborn Errors of Metabolism in Humans. Interlaken (Switzerland). September 1980."},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)88090-5"},{"key":"e_1_2_1_18_2","first-page":"256","volume-title":"Neonatal Screening","author":"Alonso\u2010Fern\u00e1ndez JR","year":"1982"},{"key":"e_1_2_1_19_2","unstructured":"Alonso\u2010Fern\u00e1ndezJR Casti\u00f1eirasC VillarP CochoJA B\u00f3vedaMD.Continuous\u2010evaporative chromatography with a discrete volumen of elution in the detection and classification of galactosemias. In: HL Levy RJ Hermos GF Grady editors. Proceedings of the Third Meeting of the International Society for Neonatal Screening Jamaica Plain MA USA. 1996. p129\u2013130."},{"key":"e_1_2_1_20_2","first-page":"149","volume-title":"Tr\u00eda neonatal de galactosemias empleando la muestra de orina. 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