{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:44:13Z","timestamp":1760298253700},"reference-count":16,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,5,13]],"date-time":"2010-05-13T00:00:00Z","timestamp":1273708800000},"content-version":"vor","delay-in-days":132,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human African trypanosomiasis (HAT), also called African sleeping sickness, is a neglected tropical parasitic disease indigenous to sub\u2010Saharan Africa. Diamidine compounds, including pentamidine and CPD\u20100801, are potent anti\u2010trypanosomal molecules. The latter is a potential drug in the development at the UNC based Consortium for Parasitic Drug Development. An orally bioavailable prodrug of CPD\u20100801, DB868, is metabolized primarily in the liver to the active form. A monoclonal antibody developed against a pentamidine derivative has shown significant reactivity with CPD\u20100801 (EC<jats:sub>50<\/jats:sub> 65.1\u2009nM), but not with the prodrug (EC<jats:sub>50<\/jats:sub>&gt;18,000\u2009nM). An inhibitory enzyme\u2010linked immunosorbent assay (IELISA) has been used to quantitatively monitor prodrug metabolism by detecting the production of the active compound over time in a sandwich culture rat hepatocyte system and in rats. These results were compared with the results of the standard LC\/MS\/MS assay. Spearman coefficients of 0.96 and 0.933 (in vitro and in vivo, respectively) indicate a high correlation between these two measurement methods. This novel IELISA provides a facile, inexpensive, and accurate method for drug detection that may aide in elucidating the mechanisms of action and toxicity of existing and future diamidine compounds. J. Clin. Lab. Anal. 24:187\u2013194, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.20380","type":"journal-article","created":{"date-parts":[[2010,5,14]],"date-time":"2010-05-14T14:46:52Z","timestamp":1273848412000},"page":"187-194","source":"Crossref","is-referenced-by-count":4,"title":["Application of monoclonal antibodies to measure metabolism of an anti\u2010trypanosomal compound in vitro and in vivo"],"prefix":"10.1002","volume":"24","author":[{"given":"Rachel Beaulieu","family":"Goldsmith","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danny R.","family":"Gray","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhixia","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia N.","family":"Generaux","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard R.","family":"Tidwell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Howard M.","family":"Reisner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,5,13]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.1.133-146.2005"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2006.01.012"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0707354"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.21429"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00831-08"},{"key":"e_1_2_1_7_2","volume-title":"Phase 3 Trial of Pafuramidine Maleate (Db289), A Novel, Oral Drug, for Treatment of First Stage Sleeping Sickness: Safety and Efficacy","author":"Pholig G","year":"2008"},{"key":"e_1_2_1_8_2","first-page":"20","article-title":"New treatment option for second stage African sleeping sickness: in vitro and in vivo efficacy of aza analogs of DB289","volume":"20","author":"Wenzler T","year":"2009","journal-title":"Antimicrob Agents Chemother"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2825(2000)14:2<73::AID-JCLA7>3.0.CO;2-G"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.123.4.1548"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"894","DOI":"10.4049\/jimmunol.114.2_Part_2.894","article-title":"Identification of macrophage\u2010like characteristics in a cultured murine tumor line","volume":"114","author":"Koren HS","year":"1975","journal-title":"J Immunol"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3726549"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1999.277.1.G12"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.106.013391"},{"key":"e_1_2_1_15_2","unstructured":"TrendlerKL AllenJL HallJE et al.Quantification of the prodrug DB289 and an active metabolite DB75 in rat and monkey plasma using SPE liquid chromatography and electrospray ionization tandem mass spectrometry. 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