{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T05:26:50Z","timestamp":1792301210462,"version":"4.4.0"},"reference-count":16,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,5,13]],"date-time":"2010-05-13T00:00:00Z","timestamp":1273708800000},"content-version":"vor","delay-in-days":132,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Despite brucellosis having a low incidence rate in developed nations, it still remains the leading zoonotic disease in the world. Culturing of <jats:italic>Brucella<\/jats:italic> spp. provides good specificity but in cases where the fever is intermittent, sensitivity is problematic. This has led to the development of serological methods of detection. Brucella agglutination methods have been considered the serological gold\u2010standard since their inception, although commercial Brucella IgG and IgM enzyme\u2010linked immunosorbentassays are available to potentially aid in the diagnosis of the disease. In our study, anti\u2010<jats:italic>Brucella<\/jats:italic> IgG and IgM assays were compared with agglutination. Individually the IgG assay tested had an accuracy of 56% and the IgM assay had an accuracy of 77%. These poor accuracies reinforce Centers for Disease Control's conclusion that nonagglutination tests should not be used to confirm brucellosis. J. Clin. Lab. Anal. 24:160\u2013162, 2010. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.20382","type":"journal-article","created":{"date-parts":[[2010,5,14]],"date-time":"2010-05-14T14:47:14Z","timestamp":1273848434000},"page":"160-162","source":"Crossref","is-referenced-by-count":21,"title":["A comparison of Brucella IgG and IgM ELISA assays with agglutination methodology"],"prefix":"10.1002","volume":"24","author":[{"given":"Ryan J.","family":"Welch","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine M.","family":"Litwin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,5,13]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Principles and Practice of Infectious Diseases","author":"Mandell G","year":"2004"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70382-6"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12038-008-0072-1"},{"key":"e_1_2_1_5_2","first-page":"603","article-title":"Public health consequences of a false\u2010positive laboratory test result for Brucella\u2014Florida, Georgia, and Michigan, 2005","volume":"57","author":"CDC","year":"2008","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00424-07"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(80)90027-7"},{"key":"e_1_2_1_8_2","volume-title":"Statistical Methods for Rates and Proportions","author":"Fleiss JF","year":"1981"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2006.08.017"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/13.3.359"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(03)00081-6"},{"key":"e_1_2_1_12_2","first-page":"1","article-title":"Joint FAO\/WHO expert committee on brucellosis","volume":"740","author":"WHO","year":"1986","journal-title":"World Health Organ Tech Rep Ser"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0040317"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-4-18"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1843.2001.00344.x"},{"key":"e_1_2_1_16_2","first-page":"1334","article-title":"Evaluation of the PANBIO Brucella immunoglobulin G (IgG) and IgM enzyme\u2010linked immunosorbent assays for diagnosis of human brucellosis","volume":"12","author":"Araj GF","year":"2005","journal-title":"Clin Diagn Lab Immunol"},{"key":"e_1_2_1_17_2","first-page":"1","article-title":"Case definitions for infectious conditions under public health surveillance. 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