{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T06:03:02Z","timestamp":1750658582714},"reference-count":11,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,7,22]],"date-time":"2011-07-22T00:00:00Z","timestamp":1311292800000},"content-version":"vor","delay-in-days":202,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2011,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Correlation of serologic titers for <jats:italic>Chlamydia trachomatis<\/jats:italic> with other tests has been based on direct fluorescence antibody (DFA) testing and culture, but not on nucleic acid\u2010based tests that are used for screening<jats:italic>.<\/jats:italic> We retrospectively reviewed the specificity of antibodies against <jats:italic>C. trachomatis<\/jats:italic>, <jats:italic>Chlamydia psittaci<\/jats:italic>, and <jats:italic>Chlamydophila pneumoniae<\/jats:italic> by microimmunofluorescence (MIF) when compared with DFA, culture, nucleic acid probe, and transcription\u2010mediated amplification. Over a 6\u2010year period, 226 cases had both MIF and one of these other methods performed for comparison. Agreement between <jats:italic>C. trachomatis<\/jats:italic> antigen or nucleic acid detection and MIF results was 87% (197\/226). <jats:italic>C. trachomatis<\/jats:italic> serology had a negative predictive value of 98%, and 10.6% of cases were positive by serology and negative by antigen testing. Of the 13 patients who had a positive <jats:italic>C. trachomatis<\/jats:italic> antigen or nucleic acid test result, 9 had IgG and\/or IgM titers highest against <jats:italic>C. trachomatis<\/jats:italic>, 3 had IgG titers highest against <jats:italic>C. pneumoniae<\/jats:italic>, and 1 had undetectable titers for the three chlamydial species. Twenty\u2010five patients had positive IgG and\/or IgM titers to <jats:italic>C. trachomatis<\/jats:italic> but negative antigen test results. Serologic testing can increase the sensitivity of detecting <jats:italic>C. trachomatis<\/jats:italic> infections. J. Clin. Lab. Anal. 25:305\u2013308, 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.20475","type":"journal-article","created":{"date-parts":[[2011,7,24]],"date-time":"2011-07-24T16:29:32Z","timestamp":1311524972000},"page":"305-308","source":"Crossref","is-referenced-by-count":9,"title":["Correlation of <i>Chlamydia<\/i> and <i>Chlamydophila<\/i> spp. IgG and IgM antibodies by microimmunofluorescence with antigen detection methods"],"prefix":"10.1002","volume":"25","author":[{"given":"Rosemary C.","family":"She","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan","family":"Welch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew R.","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Davis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine M.","family":"Litwin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,7,22]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199303000-00001"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.104.1.208"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.52.2.99"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199801000-00004"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s100960000397"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s150100050054"},{"key":"e_1_2_5_8_2","volume-title":"Sexually Transmitted Disease Surveillance 2008","author":"Centers for Disease Control and Prevention","year":"2009"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.56.3.225"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199209000-00004"},{"key":"e_1_2_5_11_2","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1128\/jcm.24.5.731-735.1986","article-title":"Accuracy of immunoglobulin M immunoassay for diagnosis of chlamydial infections in infants and adults","volume":"24","author":"Mahony JB","year":"1986","journal-title":"J Clin Microbiol"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02017710"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.20475","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.20475","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,9]],"date-time":"2023-10-09T14:59:20Z","timestamp":1696863560000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.20475"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,1]]},"references-count":11,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2011,1]]}},"alternative-id":["10.1002\/jcla.20475"],"URL":"https:\/\/doi.org\/10.1002\/jcla.20475","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,1]]}}}