{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T15:19:06Z","timestamp":1758122346134},"reference-count":10,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,3,29]],"date-time":"2012-03-29T00:00:00Z","timestamp":1332979200000},"content-version":"vor","delay-in-days":57,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:sec><jats:label \/><jats:p><jats:italic>Chlamydia trachomatis<\/jats:italic> (CT) and <jats:italic>Neisseria gonorrhoeae<\/jats:italic> (GC) are the two most common sexually transmitted bacterial infections in developed countries. The purpose of the present study was evaluating a new system for CT\/GC detection in urine specimens. A total of 700 urine specimens were obtained from patients attending the STD Outpatients Clinic of St. Orsola University Hospital, Bologna, Italy. Samples were tested by VERSANT\u00ae CT\/GC DNA 1.0 Assay (Siemens Healthcare Diagnostics Inc., Tarrytown, NY), a multiplex Real\u2010Time PCR assay, for simultaneous CT\/GC detection. Results obtained by VERSANT assay were compared with those obtained by culturing genital secretions of the same patients. Moreover, urine specimens testing positive in VERSANT assay were retested by in\u2010house PCR assays, used as confirmatory tests. VERSANT\u00ae CT\/GC DNA 1.0 Assay performed with 99.4% and 99.2% of specificity for GC and CT detection, respectively, whereas sensitivity was 100% both for CT and GC. Culture methods were 100% specific, but far less sensitive than VERSANT assay. VERSANT\u00ae CT\/GC DNA 1.0 Assay demonstrated to be a highly sensitive and specific technique for CT\/GC detection.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.21485","type":"journal-article","created":{"date-parts":[[2012,3,31]],"date-time":"2012-03-31T01:38:09Z","timestamp":1333157889000},"page":"70-72","source":"Crossref","is-referenced-by-count":6,"title":["Evaluation of the New Test <scp>VERSANT CT<\/scp>\/<scp>GC DNA<\/scp> 1.0 <scp>A<\/scp>ssay for the Detection of <i><scp>C<\/scp>hlamydia trachomatis<\/i> and <i><scp>N<\/scp>eisseria gonorrhoeae<\/i> in Urine Specimens"],"prefix":"10.1002","volume":"26","author":[{"given":"Antonella","family":"Marangoni","sequence":"first","affiliation":[{"name":"Section of Microbiology DESOS University of Bologna Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudio","family":"Foschi","sequence":"additional","affiliation":[{"name":"Section of Microbiology DESOS University of Bologna Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paola","family":"Nardini","sequence":"additional","affiliation":[{"name":"Section of Microbiology DESOS University of Bologna Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonietta","family":"D'Antuono","sequence":"additional","affiliation":[{"name":"Section of Dermatology Dipartimento di Med. Interna Dell'invecchiamento e Malattie nefrologiche University of Bologna Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicoletta","family":"Banzola","sequence":"additional","affiliation":[{"name":"Section of Dermatology Dipartimento di Med. Interna Dell'invecchiamento e Malattie nefrologiche University of Bologna Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonietta","family":"Di Francesco","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze Mediche Veterinarie University of Bologna Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberto","family":"Cevenini","sequence":"additional","affiliation":[{"name":"Section of Microbiology DESOS University of Bologna Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,3,29]]},"reference":[{"key":"e_1_2_5_2_1","unstructured":"2008 McGraw\u2010Hill New York WE Stamm KK Holmes PF Sparling WE Stamm JN Wasserheit L Corey MS Cohen HD Watts C hlamydia trachomatis infections of the adult 575 594"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.7.3041-3045.2004"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01446-09"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01019-10"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2006.00149.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/sti.77.6.419"},{"key":"e_1_2_5_8_1","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1128\/jcm.32.2.528-530.1994","article-title":"Improved PCR sensitivity for direct genotyping of \n                     Chlamydia trachomatis serovars by using a nested PCR","volume":"32","author":"Lan J","year":"1994","journal-title":"J Clin Microbiol"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-008-0650-z"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.45.5.439"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-51-12-1021"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.21485","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.21485","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T05:15:07Z","timestamp":1700543707000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.21485"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2]]},"references-count":10,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,2]]}},"alternative-id":["10.1002\/jcla.21485"],"URL":"https:\/\/doi.org\/10.1002\/jcla.21485","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2]]}}}