{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T06:56:58Z","timestamp":1790060218061,"version":"4.0.1"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,3,29]],"date-time":"2012-03-29T00:00:00Z","timestamp":1332979200000},"content-version":"vor","delay-in-days":57,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2012,2]]},"abstract":"Background<\/jats:title>RHD<\/jats:styled-content><\/jats:italic> alleles leading to a reduced expression of D<\/jats:styled-content> antigen of the red blood cell (RBC<\/jats:styled-content>) surface may be erroneously typed as D<\/jats:styled-content>\u2212 by serology and may cause anti\u2010D<\/jats:styled-content> immunizations when transfused to recipients.<\/jats:p><\/jats:sec>Methods<\/jats:title>To determine the occurrence of such alleles among apparent D\u2212 blood donors, molecular typing was implemented as a routine test using a pool of DNA<\/jats:styled-content>. A total of 2,450 pretyped D<\/jats:styled-content>\u2212 samples were tested in pools of 10 for the RHD<\/jats:styled-content><\/jats:italic>\u2010specific polymorphism in intron 4 and exon 7. Samples in polymer chain reaction (PCR) positive pools were individually reevaluated by exon\u2010specific PCRs, sequencing, and serologic methods.<\/jats:p><\/jats:sec>Results<\/jats:title>Among 2,450 serologically D\u2212 blood donor samples tested, 101 (4.1%) carried the RHD<\/jats:styled-content><\/jats:italic> gene. Nonfunctional RHD<\/jats:styled-content> (RHD<\/jats:styled-content><\/jats:italic>\u03c8, RHD<\/jats:styled-content>*CE(2\u20139)\u2010D<\/jats:italic>, and RHD<\/jats:styled-content>*CE(3\u20137)\u2010D)<\/jats:italic>, different weak D alleles such as RHD<\/jats:styled-content>*weak D type 1<\/jats:italic>, RHD<\/jats:styled-content>*weak D type 4.3<\/jats:italic>, RHD<\/jats:styled-content>*weak D type 5<\/jats:italic>, RHD<\/jats:styled-content>*weak D type 38<\/jats:italic>, and RHD<\/jats:styled-content>*DEL<\/jats:italic> were identified.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>We employed a PCR\u2010based assay for RHD<\/jats:styled-content><\/jats:italic> as a routine test using pools of ten DNA blood donor samples. The integration of RHD<\/jats:styled-content><\/jats:italic> genotyping into the routine screening program using pools of DNA samples was straightforward. As a consequence, 19 (0.8%) blood donors carrying a weak D and Del phenotypes with the potential of causing anti\u2010D immunizations in recipients were reclassified as D+. For each population, it would be necessary to adapt the RHD<\/jats:styled-content><\/jats:italic> genotyping strategy to the spectrum of prevalent alleles.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.21489","type":"journal-article","created":{"date-parts":[[2012,3,31]],"date-time":"2012-03-31T01:38:09Z","timestamp":1333157889000},"page":"104-108","source":"Crossref","is-referenced-by-count":23,"title":["RHD<\/scp><\/i> Allelic Identification Among D<\/scp>\u2212Brazilian Blood Donors as a Routine Test Using Pools of DNA"],"prefix":"10.1002","volume":"26","author":[{"given":"Mariza","family":"Mota","sequence":"first","affiliation":[{"name":"Department of Hemotherapy Albert Einstein Hospital S\u00e3o Paulo SP Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Dezan","sequence":"additional","affiliation":[{"name":"Department of Hemotherapy Albert Einstein Hospital S\u00e3o Paulo SP 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