{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T20:22:47Z","timestamp":1792873367765,"version":"4.5.0"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,3,29]],"date-time":"2012-03-29T00:00:00Z","timestamp":1332979200000},"content-version":"vor","delay-in-days":57,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>Elevation of homocysteine levels have been involved as a remarkable risk factor for cardiovascular disease. Decreased bioavailability of nitric oxide (<jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>) may result in abnormal reactions between the vessel wall and platelets and is thus involved in the initiation and progression of atherosclerosis. We aimed to assess the effect of a low dose oral contraceptive pills on homocysteine and <jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content> levels which may influence the individual cardiovascular risk by regulation of endothelial function and development of atherosclerosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The study was conducted in 50 healthy women with normal menstrual cycles as control group and 50 healthy women receiving oral contraceptive pill for at least three menstrual cycles. Homocysteine concentration was assayed by an enzyme immunoassay method and a colorimetric assay was used for determination of <jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content> levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After 3 months of treatment, homocysteine levels were significantly increased (<jats:italic>P<\/jats:italic>\u00a0= 0.027), and there was a significant and considerable decrease (<jats:italic>P<\/jats:italic>\u00a0= 0.048) in NO concentration of oral contraceptive pill (<jats:styled-content style=\"fixed-case\">OCP<\/jats:styled-content>) consumers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our data indicates that <jats:styled-content style=\"fixed-case\">OCP<\/jats:styled-content>s increase the homocysteine (<jats:styled-content style=\"fixed-case\">HCY<\/jats:styled-content>) and reduce the <jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content> levels. These findings warrant a possible implication of <jats:styled-content style=\"fixed-case\">OCP<\/jats:styled-content> in change of risk of development of coronary heart disease.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.21492","type":"journal-article","created":{"date-parts":[[2012,3,31]],"date-time":"2012-03-31T01:38:09Z","timestamp":1333157889000},"page":"120-123","source":"Crossref","is-referenced-by-count":24,"title":["Influence of Oral Contraceptive Pills on Homocysteine and Nitric Oxide Levels: As Risk Factors for Cardiovascular Disease"],"prefix":"10.1002","volume":"26","author":[{"given":"Soudabeh","family":"Fallah","sequence":"first","affiliation":[{"name":"Biochemistry Department of Medicine Faculty Tehran University of Medical Sciences Hemmat Highway Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vajihe","family":"Nouroozi","sequence":"additional","affiliation":[{"name":"Biochemistry Department of Medicine Faculty Tehran University of Medical Sciences Hemmat Highway Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morteza","family":"Seifi","sequence":"additional","affiliation":[{"name":"Biochemistry Department of Medicine Faculty Tehran University of Medical Sciences Hemmat Highway Tehran Iran"},{"name":"Gifted &amp; Talented Students Center Medical Education &amp; Development Center Tran University of Medical Science Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Samadikuchaksaraei","sequence":"additional","affiliation":[{"name":"Department of Medical Biotechnology Cellular and Molecular Research Center Tehran University of Medical Sciences Tehran Iran"},{"name":"Biological Systems Engineering Laboratory Center for Process Systems Engineering, Department of Chemical Engineering, Imperial College London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaheh Mokhtarned","family":"Aghdashi","sequence":"additional","affiliation":[{"name":"Mathematics Department Sharif University Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,3,29]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.contraception.2010.07.026"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/meta.2002.34038"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.metabol.2004.07.015"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.103.13.1813"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/CS20000173"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejheart.2005.11.016"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1042\/bst0260039","article-title":"Functions of nitric oxide in bone","volume":"26","author":"Hauschka PV","year":"1998","journal-title":"Biochem Soc Trans"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1046\/j.1365-2613.1999.00137.x","article-title":"Endothelial nitric oxide in humans in health and disease","volume":"80","author":"Vallance P","year":"1999","journal-title":"Int J Exp Pathol"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.96.8.2542"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000074452"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.281.19.1817"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9150(99)00321-4"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.31.2.582"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/deh700"},{"key":"e_1_2_6_16_1","first-page":"525","article-title":"Gender differences in serum homocysteine levels and associated factors","volume":"138","author":"Mayer O Jr","year":"1999","journal-title":"Cas Lek Cesk"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nutr.19.1.217"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1159\/000074452"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.95.5.1119"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1681.1999.03133.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.1983.tb00121.x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.20.6.1557"},{"issue":"9","key":"e_1_2_6_23_1","first-page":"1216","volume":"51","author":"Merki-Feld GS","year":"2002","journal-title":"Effects of two oral contraceptives on plasma levels of nitric oxide, homocysteine, and lipid metabolism"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.21492","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.21492","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T05:15:48Z","timestamp":1700543748000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.21492"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2]]},"references-count":22,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,2]]}},"alternative-id":["10.1002\/jcla.21492"],"URL":"https:\/\/doi.org\/10.1002\/jcla.21492","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2]]}}}