{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T14:32:57Z","timestamp":1774362777415,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,5,24]],"date-time":"2012-05-24T00:00:00Z","timestamp":1337817600000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2012,5]]},"abstract":"<jats:p>Specific genetic conditions are known to be associated with high risk of venous thromboembolism. This genetic basis varies widely between ethnic groups. We investigated the distribution of four inherited polymorphisms in 113 unselected <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>unisian blood donors by using polymerase chain reaction\u2010restriction fragment length polymorphism (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">RFLP<\/jats:styled-content>) method. The allele frequencies of Factor V <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>eiden (<jats:styled-content style=\"fixed-case\">FVL<\/jats:styled-content>), prothrombin 20210G&gt;<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>, methylenetetrahydrofolate reductase (<jats:styled-content style=\"fixed-case\">MTHFR<\/jats:styled-content>) 677<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>&gt;<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>, and <jats:styled-content style=\"fixed-case\">MTHFR<\/jats:styled-content> 1298<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>&gt;<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content> mutations were 3, 0.9, 30, and 31%, respectively. The <jats:styled-content style=\"fixed-case\">MTHFR<\/jats:styled-content> 677<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>&gt;<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> polymorphism was influenced by age. Twenty\u2010nine of the 113 blood donors demonstrated more than one genetic markers. Hyperhomocysteinemia was found in 12 subjects, and it was statistically associated to the <jats:styled-content style=\"fixed-case\">MTHFR<\/jats:styled-content> 677<jats:styled-content style=\"fixed-case\">TT<\/jats:styled-content> genotype. Principal component analysis allowed disclosing the resemblance between <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>editerranean populations. Our findings may be helpful for population genetics study, and provide epidemiologic database for further studies in thrombosis field among <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>unisians. J. Clin. Lab. Anal. 26:167\u2010173, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jcla.21506","type":"journal-article","created":{"date-parts":[[2012,5,24]],"date-time":"2012-05-24T18:55:00Z","timestamp":1337885700000},"page":"167-173","source":"Crossref","is-referenced-by-count":8,"title":["Factor V <scp>L<\/scp>eiden, Prothrombin 20210<scp>G<\/scp>&gt;<scp>A<\/scp>, MTHFR 677<scp>C<\/scp>&gt;<scp>T<\/scp> and 1298<scp>A<\/scp>&gt;<scp>C<\/scp>, and Homocysteinemia in <scp>T<\/scp>unisian Blood Donors"],"prefix":"10.1002","volume":"26","author":[{"given":"Samira","family":"Hadhri","sequence":"first","affiliation":[{"name":"Laboratoire d'H\u00e9matologie et Banque du Sang CHU Sahloul Sousse Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamed Ben","family":"Rejab","sequence":"additional","affiliation":[{"name":"Laboratoire d'Hygi\u00e8ne Hospitali\u00e8re CHU Sahloul Sousse Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hajer","family":"Guedria","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie et Banque du Sang CHU Sahloul Sousse Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lamia","family":"Ifa","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie et Banque du Sang CHU Sahloul Sousse Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noureddine","family":"Chatti","sequence":"additional","affiliation":[{"name":"Institut Sup\u00e9rieur de Biotechnologie Monastir Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hadef","family":"Skouri","sequence":"additional","affiliation":[{"name":"Laboratoire d'H\u00e9matologie et Banque du Sang CHU Sahloul Sousse Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,5,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2007.02502.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2009.03394.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2007.02489.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-009-0447-6"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymgme.2005.04.002"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2009.07786.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.20733"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/jhg.2010.11"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2008.12.033"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.14.4440"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2005.01141.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"crossref","first-page":"587","DOI":"10.18388\/abp.2008_3065","article-title":"Methylenetetrahydrofolate reductase (MTHFR\u2010677 and MTHFR\u20101298) genotypes and haplotypes and plasma homocysteine levels in patients with occlusive artery disease and deep venous thrombosis","volume":"55","author":"Spiroski I","year":"2008","journal-title":"Acta Biochim Pol"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH10-08-0510"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199909093411104"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.17.9.1662"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.109.906347"},{"key":"e_1_2_7_18_1","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1160\/TH06-09-0486","article-title":"No interaction between Factor V Leiden and hyperhomocysteinemia or MTHFR 677TT genotype in venous thrombosis. 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