{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T01:27:28Z","timestamp":1781832448890,"version":"3.54.5"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,5,24]],"date-time":"2012-05-24T00:00:00Z","timestamp":1337817600000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2012,5]]},"abstract":"NMO<\/jats:styled-content>\u2010I<\/jats:styled-content>gG<\/jats:styled-content>against aquaporin\u20104 (AQP<\/jats:styled-content>4) is a specific marker for neuromyelitis optica (NMO<\/jats:styled-content>). We evaluated the performance of different NMO<\/jats:styled-content>\u2010I<\/jats:styled-content>gG<\/jats:styled-content> detecting methods. In 124 sera (from 54 with NMO<\/jats:styled-content> spectrum disorders including nine with NMO<\/jats:styled-content>, ten with multiple sclerosis including two with OSMS<\/jats:styled-content>, and 60 with other neurological diseases), NMO<\/jats:styled-content>\u2010I<\/jats:styled-content>gG<\/jats:styled-content> was measured with tissue\u2010based indirect immunofluorescence (IIF<\/jats:styled-content>\u2010tissue) using mouse cerebellum, cell\u2010based IIF<\/jats:styled-content> (IIF<\/jats:styled-content>\u2010AQP<\/jats:styled-content>4) using transfected HEK<\/jats:styled-content>293 cells which express human AQP<\/jats:styled-content>4, and AQP<\/jats:styled-content>4 autoantibody detecting enzyme linked immunosorbent assay (ELISA<\/jats:styled-content>\u2010AQP<\/jats:styled-content>4). The sensitivities and specificities of three assays were 44.4\u201355.6% and 87.0\u201392.2% for detecting NMO<\/jats:styled-content>, and 11.1\u201320.4% and 95.7\u201397.1% for detecting NMO<\/jats:styled-content> spectrum disorders. Although there was no significant difference, the patients with NMO<\/jats:styled-content> or NMO<\/jats:styled-content> spectrum disorders showed higher rates of seropositivity in the ELISA<\/jats:styled-content>\u2010AQP<\/jats:styled-content>4 vs. IIF<\/jats:styled-content> assays. Out of the 19 sera with NMO<\/jats:styled-content>\u2010I<\/jats:styled-content>gG<\/jats:styled-content>, in at least one test, only six (31.6%) were found to be positive by all three assays. Among the three methods, the ranges of co\u2010negativities, co\u2010positivities, and agreement were 77.4\u201397.4%, 42.9\u201375.0%, and 91.1\u201395.2% (kappa 0.475\u20130.641), respectively. In patients who had positive ELISA<\/jats:styled-content>\u2010AQP<\/jats:styled-content>4 results, IIF<\/jats:styled-content>\u2010AQP<\/jats:styled-content>4 positivity was associated with NMO<\/jats:styled-content> (P<\/jats:italic> = 0.01). In summary, we observed an increased prevalence of NMO<\/jats:styled-content>\u2010I<\/jats:styled-content>gG<\/jats:styled-content> in patients with NMO<\/jats:styled-content> and NMO<\/jats:styled-content> spectrum disorders. ELISA<\/jats:styled-content>\u2010AQP<\/jats:styled-content>4 may be more sensitive and specific when confirmed by IIF<\/jats:styled-content>\u2010AQP<\/jats:styled-content>4. J. Clin. Lab. Anal. 26:184\u2010189, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p>","DOI":"10.1002\/jcla.21508","type":"journal-article","created":{"date-parts":[[2012,5,24]],"date-time":"2012-05-24T18:55:00Z","timestamp":1337885700000},"page":"184-189","source":"Crossref","is-referenced-by-count":19,"title":["Detection of Anti\u2010Aquaporin\u20104 Antibodies in Neuromyelitis Optica: Comparison of Tissue\u2010Based and Cell\u2010Based Indirect Immunofluorescence Assays and ELISA<\/scp>"],"prefix":"10.1002","volume":"26","author":[{"given":"Yoon\u2010Joo","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung\u2010Won","family":"Jung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yonggoo","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeon\u2010Joon","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyungja","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun\u2010Jee","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine School of Medicine The Catholic University of Korea Seoul Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,5,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(07)70216-8"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.53.5.1107"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/WCO.0b013e32814f1c6b"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2010.10.012"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-004-0271-0"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11940-005-0010-6"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)61620-7"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/1352458506070732"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.WNL.0000159399.81861.D5"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.65.11.noc80069"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.51.4.1219"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)17551-X"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20050304"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000216139.44259.74"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jns.2010.01.002"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1177\/1352458509106851"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2008.03.009"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-2094-7-50"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.20703"},{"key":"e_1_2_7_21_1","first-page":"99","article-title":"Detection of anti\u2010aquaporin\u20104 antibodies in neuromyelitis optica: Current status of the assays","volume":"15","author":"Waters P","year":"2008","journal-title":"Int MS J"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/1352458507084595"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-007-0509-8"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp.2005.080390"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(03)00308-9"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1177\/1352458506071174"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.21508","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.21508","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T09:09:37Z","timestamp":1698656977000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.21508"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,5]]},"references-count":25,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012,5]]}},"alternative-id":["10.1002\/jcla.21508"],"URL":"https:\/\/doi.org\/10.1002\/jcla.21508","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,5]]}}}