{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T12:24:48Z","timestamp":1763036688303},"reference-count":16,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,7,18]],"date-time":"2012-07-18T00:00:00Z","timestamp":1342569600000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2012,7]]},"abstract":"Introduction<\/jats:title>Archival tissue samples preserved in formalin are a great source of treasure for biomedical research and diagnostics. Formalin, though is a good preservative, causes the modification of nucleic acid limiting the application of fixed tissues. The present study evaluated three methods of RNA<\/jats:styled-content>extraction for constitutive gene expression and pathogen detection.<\/jats:p><\/jats:sec>Material and methods<\/jats:title>Sixteen archival formalin\u2010fixed paraffin\u2010embedded (FFPE<\/jats:styled-content>) myocardial tissues were subjected to RNA<\/jats:styled-content>extraction by Trizol, SDS<\/jats:styled-content>, and RN<\/jats:styled-content>easy FFPE<\/jats:styled-content>kit followed by RT<\/jats:styled-content>\u2010PCR<\/jats:styled-content>and Taqman Real\u2010Time PCR<\/jats:styled-content>to study the expression of housekeeping genes.<\/jats:p><\/jats:sec>Results<\/jats:title>RNA<\/jats:styled-content>was extracted from all 16 myocardial tissues (100%) by RN<\/jats:styled-content>easy FFPE<\/jats:styled-content>kit, as compared to 14\/16 by Trizol and 8\/10 by SDS<\/jats:styled-content>methods. The expression of Glyceraldehye\u20103\u2010phosphate dehydrogenase (GAPDH)<\/jats:styled-content>was observed in RNA<\/jats:styled-content>extracted by RN<\/jats:styled-content>easy FFPE<\/jats:styled-content>kit and Trizol. High yield of RNA<\/jats:styled-content>was obtained by RN<\/jats:styled-content>easy FFPE<\/jats:styled-content>kit than Trizol (P<\/jats:italic>\u00a0=\u00a00.002) and SDS<\/jats:styled-content>(P\u00a0<\/jats:italic>=\u00a00.012). Of the three methods, RN<\/jats:styled-content>easy FFPE<\/jats:styled-content>kit was evaluated for Enterovirus RNA<\/jats:styled-content>detection in 16 other histopathologically confirmed FFPE<\/jats:styled-content>tissues of dilated cardiomyopathy (DCM<\/jats:styled-content>) cases and Enterovirus genome was detected in 4\/16 (25%) FFPE<\/jats:styled-content>tissues of DCM<\/jats:styled-content>cases. The enteroviral sequences of the viral isolates revealed 99% homology with Human coxsackievirus B<\/jats:styled-content>5.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>The Qiagen RN<\/jats:styled-content>easy FFPE<\/jats:styled-content>kit resulted in significantly high reproducibility of RNA<\/jats:styled-content>from FFPE<\/jats:styled-content>myocardial tissues, which are suitable for amplification by T<\/jats:styled-content>aq\u2010M<\/jats:styled-content>an R<\/jats:styled-content>eal\u2010T<\/jats:styled-content>ime and RT<\/jats:styled-content>\u2010PCR<\/jats:styled-content>. Thus, the results show that these FFPE<\/jats:styled-content>tissues can be used for gene expression, pathogen detection, and epidemiological studies. J. Clin. Lab. Anal. 26:279\u2010285, 2012. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.21518","type":"journal-article","created":{"date-parts":[[2012,7,19]],"date-time":"2012-07-19T08:44:41Z","timestamp":1342687481000},"page":"279-285","source":"Crossref","is-referenced-by-count":12,"title":["Ribonucleic Acid Extraction From Archival Formalin Fixed Paraffin Embedded Myocardial Tissues for Gene Expression and Pathogen Detection"],"prefix":"10.1002","volume":"26","author":[{"given":"Mirnalini","family":"Sharma","sequence":"first","affiliation":[{"name":"Department of Virology Postgraduate Institute of Medical Education and Research Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baijayantimala","family":"Mishra","sequence":"additional","affiliation":[{"name":"Department of Virology Postgraduate Institute of Medical Education and Research Chandigarh 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