{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T07:42:27Z","timestamp":1698738147360},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,11,9]],"date-time":"2012-11-09T00:00:00Z","timestamp":1352419200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Hepatitis <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> virus (<jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content>) is one of the leading causes of acute and chronic liver diseases and in turn responsible for a million of worldwide annual deaths. <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content>has been classified into eight main groups, designated as <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content> with different geographical distribution. Some genotypes are associated with different clinical outcomes and particular viral mutations. Current study was aimed to investigate the genotype and prevalence of <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content> in blood donors of south of <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>ran.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This experimental study investigated the prevalence of <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content>positivity in 198,289 <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>ranian blood donors from south of <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>ran by both <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>\u2010 and <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010based methods. Within 198,289 donors, 120 <jats:styled-content style=\"fixed-case\">HB<\/jats:styled-content>s<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>g<jats:sup>+<\/jats:sup> cases were selected, <jats:styled-content style=\"fixed-case\">HBV\u2010DNA<\/jats:styled-content>was extracted, and the p gene sequences were amplified by nested\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. The <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content>genotypes were determined by direct sequencing of the polymerase gene of <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content>. Phylogenetic trees also were constructed by the neighbor\u2010joining (<jats:styled-content style=\"fixed-case\">NJ<\/jats:styled-content>) method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Findings of this study indicated that 0.184%, 0.329%, and 0.215% of blood donors were <jats:styled-content style=\"fixed-case\">HB<\/jats:styled-content>s<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>g<jats:sup>+<\/jats:sup> among <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>sfahan, <jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>erman, and <jats:styled-content style=\"fixed-case\">Y<\/jats:styled-content>azd provinces, respectively. Only 69 (57.5%) cases of 120 <jats:styled-content style=\"fixed-case\">HB<\/jats:styled-content>s<jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>g<jats:sup>+<\/jats:sup> donors were <jats:styled-content style=\"fixed-case\">HBV\u2010DNA<\/jats:styled-content><jats:sup>+<\/jats:sup>. Sequencing analysis revealed that all of <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content>\u2010infected donors had the <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content> genotype of <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In conclusion, the prevalence of genotype <jats:styled-content style=\"fixed-case\">D<\/jats:styled-content> was 100% in <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>ranian <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content>blood donors. These findings may have an impact on the immunological and genetic diagnosis of <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content>, selection of diagnostic kits, and viral quality control panels to evaluate diagnostic methods.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.21534","type":"journal-article","created":{"date-parts":[[2012,11,9]],"date-time":"2012-11-09T23:23:15Z","timestamp":1352503395000},"page":"407-411","source":"Crossref","is-referenced-by-count":1,"title":["Study of Hepatitis <scp>B<\/scp> Prevalence in Parallel With the Most Frequent <scp>HBV<\/scp>Genotype in South <scp>I<\/scp>ranian Blood Donors"],"prefix":"10.1002","volume":"26","author":[{"given":"Mojgan Noroozi","family":"Karimabad","sequence":"first","affiliation":[{"name":"Molecular Medicine Research Center Rafsanjan University of Medical Sciences Rafsanjan Iran"},{"name":"Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran 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