{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T05:13:33Z","timestamp":1784178813961,"version":"3.55.0"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2012,11,9]],"date-time":"2012-11-09T00:00:00Z","timestamp":1352419200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2012,11]]},"abstract":"The authors conducted a flow cytometry immunophenotyping study in patients with acute lymphoblastic leukemia (ALL<\/jats:styled-content>) from N<\/jats:styled-content>atal, R<\/jats:styled-content>io G<\/jats:styled-content>rande do N<\/jats:styled-content>orte, B<\/jats:styled-content>razil. The patients (n<\/jats:italic> = 126) were newly diagnosed using a panel of monoclonal antibodies: CD<\/jats:styled-content>1a, CD<\/jats:styled-content>2, CD<\/jats:styled-content>3, CD<\/jats:styled-content>4, CD<\/jats:styled-content>7, CD<\/jats:styled-content>8, CD<\/jats:styled-content>10, CD<\/jats:styled-content>13, CD<\/jats:styled-content>33, CD<\/jats:styled-content>14, CD<\/jats:styled-content>19, CD<\/jats:styled-content>22, CD<\/jats:styled-content>79a, CD<\/jats:styled-content>117, CD<\/jats:styled-content>34, anti\u2010I<\/jats:styled-content>gM<\/jats:styled-content>, anti\u2010T<\/jats:styled-content>dT<\/jats:styled-content>, anti\u2010HLA<\/jats:styled-content>\u2010D<\/jats:styled-content>r, and anti\u2010human kappa and lambda light chains. Additional data, such as patients' age and gender, clinical and laboratory findings such as presence of tumor masses, lymphadenopathy, hepatomegaly, splenomegaly, leukemic infiltration in the central nervous system (CNS<\/jats:styled-content>) were also investigated. Results showed that 56.7% of the cases were B<\/jats:styled-content>\u2010lineage ALL<\/jats:styled-content> and 55% were T<\/jats:styled-content>\u2010cell ALL<\/jats:styled-content>. Also, we found that males were more affected by the disease, regardless of immunological classification. The correlation between age and immunological subtypes showed that the B<\/jats:styled-content>\u2010lineage ALL<\/jats:styled-content> occurred more frequently in patients aged under 15while the T<\/jats:styled-content>\u2010cell ALL<\/jats:styled-content> subtype was more frequent in adults. Immunophenotypic profiles and morphological subtypes showed a direct correlation between L3 subtype and B<\/jats:styled-content>\u2010lineage ALL<\/jats:styled-content>, while L<\/jats:styled-content>1 and L<\/jats:styled-content>2 subtypes correlated more often with B<\/jats:styled-content>\u2010cell lineage and T<\/jats:styled-content>\u2010cell ALL<\/jats:styled-content>, respectively. Correlation analysis between immunophenotypic and clinical profiles showed that T<\/jats:styled-content>\u2010cell ALL<\/jats:styled-content> was more associated with a higher incidence of lymphadenopathy, hepatomegaly, splenomegaly and CNS<\/jats:styled-content> leukemic infiltration, also showing a greater blast cell count in peripheral blood than the other subgroups. The presented data suggest that immunophenotyping is an important method in the diagnosis, monitoring and prognostic assessment in determining the pathological mechanisms of evolution of ALL<\/jats:styled-content>.<\/jats:p>","DOI":"10.1002\/jcla.21540","type":"journal-article","created":{"date-parts":[[2012,11,9]],"date-time":"2012-11-09T23:23:15Z","timestamp":1352503395000},"page":"431-440","source":"Crossref","is-referenced-by-count":14,"title":["Flow Cytometry Immunophenotyping Evaluation in Acute Lymphoblastic Leukemia: Correlation to Factors Affecting Clinic Outcome"],"prefix":"10.1002","volume":"26","author":[{"given":"Gabriela","family":"Vasconcelos de Andrade Alves","sequence":"first","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas e Toxicol\u00f3gicas Faculdade de Farm\u00e1cia da Universidade Federal do Rio Grande do Norte Natal\u2010RN Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea Luciana","family":"Ara\u00fajo da Cunha Fernandes","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas e Toxicol\u00f3gicas Faculdade de Farm\u00e1cia da Universidade Federal do Rio Grande do Norte Natal\u2010RN Brazil"},{"name":"Laborat\u00f3rio de Hematologia, Hemocentro Dalton Cunha (HEMONORTE) Natal\u2010RN Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana Mendon\u00e7a","family":"Freire","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas e Toxicol\u00f3gicas Faculdade de Farm\u00e1cia da Universidade Federal do Rio Grande do Norte Natal\u2010RN Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aldair","family":"de Souza Paiva","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas e Toxicol\u00f3gicas Faculdade de Farm\u00e1cia da Universidade Federal do Rio Grande do Norte Natal\u2010RN Brazil"},{"name":"Laborat\u00f3rio de Hematologia, Hemocentro Dalton Cunha (HEMONORTE) Natal\u2010RN Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto Chaves","family":"de Vasconcelos","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas e Toxicol\u00f3gicas Faculdade de Farm\u00e1cia da Universidade Federal do Rio Grande do Norte Natal\u2010RN Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9ria","family":"Soraya de Farias Sales","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas e Toxicol\u00f3gicas Faculdade de Farm\u00e1cia da Universidade Federal do Rio Grande do Norte Natal\u2010RN Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Telma","family":"Maria de Ara\u00fajo Moura Lemos","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas e Toxicol\u00f3gicas Faculdade de Farm\u00e1cia da Universidade Federal do Rio Grande do Norte Natal\u2010RN Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica Aires","family":"Gil","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas e Toxicol\u00f3gicas Faculdade de Farm\u00e1cia da Universidade Federal do Rio Grande do Norte Natal\u2010RN Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1vio Henrique","family":"Miranda de Ara\u00fajo Freire","sequence":"additional","affiliation":[{"name":"Departamento de Estat\u00edstica da Universidade Federal do Rio Grande do Norte Natal\u2010RN Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dany Geraldo Kramer Cavalcanti e","family":"Silva","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias da Sa\u00fade do Trair\u00ed\u2010 (FACISA) Universidade Federal do Rio Grande do Norte Natal\u2010RN Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James Farley Rafael","family":"Maciel","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Hematologia, Hemocentro Dalton Cunha (HEMONORTE) Natal\u2010RN Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irian Guedes","family":"Farkatt","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Hematologia, Hemocentro Dalton Cunha (HEMONORTE) Natal\u2010RN Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geraldo Barroso Cavalcanti","family":"J\u00fanior","sequence":"additional","affiliation":[{"name":"Departamento de An\u00e1lises Cl\u00ednicas e Toxicol\u00f3gicas Faculdade de Farm\u00e1cia da Universidade Federal do Rio Grande do Norte Natal\u2010RN Brazil"},{"name":"Laborat\u00f3rio de Hematologia, Hemocentro Dalton Cunha (HEMONORTE) Natal\u2010RN Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,11,9]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra023001"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60457-2"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/beha.2002.0224"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2009.04.009"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1976.tb03563.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1981.tb02684.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(00)00228-3"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20112"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2009\/674801"},{"key":"e_1_2_6_11_1","first-page":"1783","article-title":"Proposals for the immunological classification of acute leukemias. European Group for the Immunological Characterization of Leukemias (EGIL)","volume":"10","author":"Bene MC","year":"1995","journal-title":"Leukemia"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.48.3.194"},{"issue":"10","key":"e_1_2_6_13_1","doi-asserted-by":"crossref","first-page":"1908\u20141923.","DOI":"10.1182\/blood.V76.10.1908.bloodjournal76101908","article-title":"Regulation of human B\u2010cell ontogeny","volume":"76","author":"Uckun UF","year":"1990","journal-title":"Blood"},{"issue":"2","key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1182\/blood.V82.2.343.343","article-title":"Clinical and biological relevance of immunologic marker studies in childhood acute lymphoblastic leukemia","volume":"82","author":"Pui CH","year":"1993","journal-title":"Blood"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.3.1588"},{"issue":"8","key":"e_1_2_6_16_1","doi-asserted-by":"crossref","first-page":"2863","DOI":"10.1182\/blood.V90.8.2863","article-title":"Recent advances in flow cytometry application to the diagnosis of hematologic malignancy","volume":"90","author":"Jenning CD","year":"1997","journal-title":"Blood"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-11-120535"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.5858\/2003-127-42-FCAOA"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.thj.6200013"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.47.9.843"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.b.20514"},{"issue":"11","key":"e_1_2_6_22_1","first-page":"967","article-title":"Clinical relevance of CD10 expression in childhood ALL. The Italian Association for Pediatric Hematology and Oncology (AIEOP)","volume":"83","author":"Consolini R","year":"1998","journal-title":"Haematologica"},{"key":"e_1_2_6_23_1","first-page":"1406","article-title":"Poor prognosis of prethymic phenotype in acute lymphoblastic leukemia","volume":"8","author":"Digel W","year":"1994","journal-title":"Leukemia"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2494(94)80027-8"},{"key":"e_1_2_6_25_1","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1182\/blood.V73.2.381.381","article-title":"CD7+\\CD4\u2010\\CD8\u2010acute leukemia: A syndrome of malignant pluripotent lymphohematopoietic cells","volume":"73","author":"Kurtzberg J","year":"1989","journal-title":"Blood"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/00043426-198921000-00015"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(87)90756-X"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-5728(94)90155-4"},{"key":"e_1_2_6_29_1","first-page":"2259","article-title":"CD44 expression in T cell lymphoblastic leukemia","volume":"27","author":"Cavalcanti GB","year":"1994","journal-title":"Braz J Med Biol Res"},{"issue":"84","key":"e_1_2_6_30_1","doi-asserted-by":"crossref","first-page":"1249","DOI":"10.1182\/blood.V84.4.1249.1249","article-title":"High levels of shed form of L\u2010selectin are present in patients with acute leukemia and inhibit blast cell adhesion to activation endothelium","volume":"15","author":"Spertini O","year":"1994","journal-title":"Blood"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428190109097660"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.2169\/internalmedicine.41.34"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1159\/000203657"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.21540","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.21540","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T19:17:30Z","timestamp":1698693450000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.21540"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11]]},"references-count":32,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["10.1002\/jcla.21540"],"URL":"https:\/\/doi.org\/10.1002\/jcla.21540","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11]]}}}