{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T17:31:46Z","timestamp":1771954306439,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,1,16]],"date-time":"2013-01-16T00:00:00Z","timestamp":1358294400000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Acne vulgaris is a multifactorial skin disorder of unknown etiology. Free radical\u2010mediated reactions have been implicated but their role in eliciting this response and contributing to disease progress remains unexplored. This study was undertaken to investigate the status and contribution of oxidative\/nitrosative stress in patients with acne vulgaris. J. Clin. Lab. Anal. 27:45\u20132, 2013. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sera from 50 acne vulgaris with varying levels of disease activity (mild, moderate, and severe) according to the <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>lobal <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>cne <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>rading <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>ystem (<jats:styled-content style=\"fixed-case\">GAGS<\/jats:styled-content>) and 40 age\u2010 and sex\u2010matched controls were evaluated for serum levels of oxidative\/nitrosative stress markers, including protein oxidation, lipid peroxidation and nitric oxide (NO), superoxide dismutase (<jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content>), and glutathione (<jats:styled-content style=\"fixed-case\">GSH<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Serum analysis showed significantly higher levels of carbonyl contents, malondialdehyde (<jats:styled-content style=\"fixed-case\">MDA<\/jats:styled-content>) and NO, in acne patients compared with healthy controls (<jats:italic>P<\/jats:italic> &lt; 0.05). Interestingly, not only there were an increased number of subjects positive for carbonyl contents, but also the levels of these oxidants were significantly increased with the increase of the disease activity (<jats:italic>P<\/jats:italic> &lt; 0.05). In addition, a significant correlation was observed between the levels of carbonyl contents and the <jats:styled-content style=\"fixed-case\">GAGS<\/jats:styled-content> scores (<jats:italic>r<\/jats:italic> = 0.341, <jats:italic>r<\/jats:italic> = 0.355, and <jats:italic>r<\/jats:italic> = 0.299, respectively). Furthermore, sera from acne patients had lower levels of <jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">GSH<\/jats:styled-content> compared with healthy control sera.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings support an association between oxidative\/nitrosative stress and acne. The stronger response observed in serum samples from patients with higher <jats:styled-content style=\"fixed-case\">GAGS<\/jats:styled-content> scores suggests that markers of oxidative\/nitrosative stress may be useful in evaluating the progression of acne and in elucidating the mechanisms of disease pathogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.21560","type":"journal-article","created":{"date-parts":[[2013,1,16]],"date-time":"2013-01-16T17:56:29Z","timestamp":1358358989000},"page":"45-52","source":"Crossref","is-referenced-by-count":31,"title":["Biochemical Markers of Oxidative and Nitrosative Stress in Acne Vulgaris: Correlation With Disease Activity"],"prefix":"10.1002","volume":"27","author":[{"given":"Hani A.","family":"Al\u2010Shobaili","sequence":"first","affiliation":[{"name":"Department of Dermatology College of Medicine Qassim University  Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdullateef A.","family":"Alzolibani","sequence":"additional","affiliation":[{"name":"Department of Dermatology College of Medicine Qassim University  Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmad A.","family":"Al Robaee","sequence":"additional","affiliation":[{"name":"Department of Dermatology College of Medicine Qassim University  Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdel\u2010Raheim M.A.","family":"Meki","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry College of Medicine Qassim University  Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zafar","family":"Rasheed","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry College of Medicine Qassim University  Kingdom of Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,1,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/156720111797635540"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2230.2011.04218.x"},{"key":"e_1_2_7_4_1","first-page":"5","article-title":"Combination topical therapy in the treatment of acne","volume":"78","author":"Del Rosso JQ","year":"2006","journal-title":"Cutis"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.25011\/cim.v34i3.15189"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1081\/DMR-100102336"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R700019200"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2005.05.006"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568026013394822"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)01222-1"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(95)93872-4"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0188-4409(02)00406-X"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M400442-JLR200"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbio.201100073"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1473-2165.2011.00570.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2009.03505.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1155\/MI.2005.380"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2011.01848.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.2174\/138945011796150334"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-4362.1997.00099.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(94)33040-9"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-8981(03)00003-2"},{"key":"e_1_2_7_23_1","first-page":"2259","article-title":"Serum lipid peroxides in rats treated chronically with adriamycin","volume":"33","author":"Thayer WS","year":"1984","journal-title":"Pharmacol"},{"key":"e_1_2_7_24_1","doi-asserted-by":"crossref","first-page":"2407","DOI":"10.4049\/jimmunol.141.7.2407","article-title":"Release of reactive nitrogen intermediates and reactive oxygen intermediate from mouse peritoneal macrophages. 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