{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T08:38:09Z","timestamp":1784191089276,"version":"3.55.0"},"reference-count":19,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,2,19]],"date-time":"2013-02-19T00:00:00Z","timestamp":1361232000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2013,3]]},"abstract":"Background<\/jats:title>Respiratory pathogens are a leading cause of hospital admission and traditional detection methods are time consuming and insensitive. Multiplex molecular detection methods have recently been investigated in hope of replacing these traditional techniques with rapid panel\u2010based testing.<\/jats:p><\/jats:sec>Objectives<\/jats:title>This study evaluated the F<\/jats:styled-content>ilmA<\/jats:styled-content>rray\u00ae<\/jats:sup> R<\/jats:styled-content>espiratory P<\/jats:styled-content>anel ([FARP<\/jats:styled-content>], Idaho Technology Inc., Salt Lake City, UT<\/jats:styled-content>) as a replacement for direct fluorescent antibody (DFA<\/jats:styled-content>) testing in a pediatric hospital.<\/jats:p><\/jats:sec>Methods<\/jats:title>Eleven of the 21 FARP<\/jats:styled-content> analytes (Adenovirus, B<\/jats:styled-content>ordetella pertussis<\/jats:italic>, human M<\/jats:styled-content>etapneumovirus, I<\/jats:styled-content>nfluenza A, I<\/jats:styled-content>nfluenza A H<\/jats:styled-content>1N<\/jats:styled-content>1 2009, I<\/jats:styled-content>nfluenza B, P<\/jats:styled-content>arainfluenza [1, 2, & 3], R<\/jats:styled-content>espiratory S<\/jats:styled-content>yncytial V<\/jats:styled-content>irus, and rhinovirus) were evaluated using nasopharyngeal specimens. Positive samples were pooled in groups of 5. Samples identified by reference methods as positive for respiratory pathogens were used for the majority of positive samples. DFA<\/jats:styled-content> was the reference method for ten analytes; L<\/jats:styled-content>uminexTM<\/jats:sup> xTAG R<\/jats:styled-content>espiratory V<\/jats:styled-content>irus P<\/jats:styled-content>anel (RVP<\/jats:styled-content>) was the reference method for rhinovirus. Discrepant results were resolved by positive culture and fluorescent antibody stain and\/or laboratory\u2010developed real\u2010time polymerase chain reaction (PCR<\/jats:styled-content>) assays (LDT<\/jats:styled-content>).<\/jats:p><\/jats:sec>Results<\/jats:title>The agreement for most analytes was in concordance with the established reference methods with the exception of A<\/jats:styled-content>denovirus. Additionally, the FARP<\/jats:styled-content> detected several pathogens not previously detected by DFA<\/jats:styled-content>, and most were confirmed by LDT<\/jats:styled-content>. Several DFA<\/jats:styled-content>\u2010positive analytes were confirmed as true\u2010negatives by the FARP and LDT<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>FARP<\/jats:styled-content> overall performed better than DFA<\/jats:styled-content> with the exception of A<\/jats:styled-content>denovirus, making the FARP<\/jats:styled-content> an attractive alternative to laboratories looking to replace DFA<\/jats:styled-content> with a rapid, user\u2010friendly, multiplex molecular assay. J. Clin. Lab. Anal. 27:148\u2013154, 2013. \u00a9 2013 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.21576","type":"journal-article","created":{"date-parts":[[2013,2,19]],"date-time":"2013-02-19T23:20:41Z","timestamp":1361316041000},"page":"148-154","source":"Crossref","is-referenced-by-count":46,"title":["Evaluation of the FilmArray\u00ae<\/sup> Respiratory Panel for Clinical Use in a Large Children's Hospital"],"prefix":"10.1002","volume":"27","author":[{"given":"Marc Roger","family":"Couturier","sequence":"first","affiliation":[{"name":"Department of Pathology University of Utah Salt Lake City Utah"},{"name":"ARUP Institute for Clinical and Experimental Pathology Salt Lake City Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trenda","family":"Barney","sequence":"additional","affiliation":[{"name":"Primary Children's Medical Center Salt Lake City Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garrison","family":"Alger","sequence":"additional","affiliation":[{"name":"Primary Children's Medical Center Salt Lake City Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weston C.","family":"Hymas","sequence":"additional","affiliation":[{"name":"ARUP Institute for Clinical and Experimental Pathology Salt Lake City Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffery B.","family":"Stevenson","sequence":"additional","affiliation":[{"name":"ARUP Institute for Clinical and Experimental Pathology Salt Lake City Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Hillyard","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Utah Salt Lake City Utah"},{"name":"ARUP Institute for Clinical and Experimental Pathology Salt Lake City Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judy A.","family":"Daly","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Utah Salt Lake City Utah"},{"name":"Primary Children's Medical Center Salt Lake City Utah"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,2,19]]},"reference":[{"issue":"3","key":"e_1_2_7_2_1","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1128\/JCM.38.3.1191-1195.2000","article-title":"Detection and identification of human parainfluenza viruses 1, 2, 3, and 4 in clinical samples of pediatric patients by multiplex reverse transcription\u2010PCR","volume":"38","author":"Aguilar JC","year":"2000","journal-title":"J Clin 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